Fang Ji

Last Updated: September 28 2022

Stephania tetrandra (Fang Ji) is a medicinal herb from Traditional Chinese Medicine that is sometimes used alongside Astragalus membranaceus for kidney protection and diabetes prevention; it is moderately antiinflammatory.

Fang Ji is most often used for.



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1.

Sources and Composition

1.1

Sources

Stephania tetrandra (of the family Menispermaceae) is a traditional chinese medicine that is also used in Japanese traditional medicine (Kampo), bearing the name hangfangji in Chinese medicine.[1] It is traditionally used against autoimmune diseases and rheumatism,[2] as well as antipyretic, diuretic, antiphlogistic, anti-diabetic as well as therapy for hepatic fibrosis.[3]

For medicinal usage, stephania tetrandra is sometimes referred to as 'Fangchi' or 'Fangji'; a term that also applies to the plants Sinomenium acutum, Cocculus trilobus, and Aristolochia fangchi[4][5] although cocculus trilobus and aristolochia fanchi are further classified as "Mu Fangchi" and "Guang Fangchi".[4]

1.2

Composition

  • The bis-benzylisoquinoline alkaloid Fangchinoline at 43.50+/-0.024mg/g in the root[6] although lower concentrations of 3.14-8.08mg/g,[4] 0.40%,[7] or 0.97-2.37%[8] have been reported
  • Tetrandrine (another bis-benzylisoquinoline alkaloid[9]) at 82.31+/-0.003mg/g of the root[6] although lower concentrations of 2.80-10.31mg/g,[4] 0.78%,[7] or 1.28-2.84%[8] have been reported. Other similar structures include 2-N-methyltetrandrine and (+)-2-N-merhylfangchinoline[10]
  • Cassameridine and cassythicine[3]
  • Corydione[3]
  • Nantenine and Oxonantenine[3]
  • Aristolochic acid I and II (potential contaminants from Aristolochia fangchi)[11]
  • Oblongine (2.90+/-0.024mg/g of the root)[6]
  • Cyclanoline (59.09+/-0.009mg/g of the root)[6]
  • Magnoflorine (140-580µg/g[4])
  • A 4,5-Dioxoaporphine alkaloid (aerial parts)[12]
  • The biflavones Stephaflavone A-B (aerial parts)[13]
  • β-sitosterol (aerial parts)[13]

Although it is subject to variance, some reports suggest that the total alkaloid content of the dry root of stephania tetrandra is approximately 2.3% of which tetrandrine and fangchinoline constitute 1% and 0.5% (total weight), respectively.[9][14]

The main bioactives appears to be the two bis-benzylisoquinoline alkaloids, fangchinoline and tetrandrine

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1.3

Formulations

The formulation known as Boi-ogi-to (Fang-ji-huang-qi-tang) is a Kampo medicine containing this herb alongside astragalus membranaceus, the rhizome of Atractylodes Lancea, licorice, ginger, and ziziphus jujuba.[15] This formulation appears to be recommended for the treatment of arthritis and edema, and may have indirect effects on glucose and lipid metabolism associated with obesity.[15]

2.

Pharmacology

2.1

Enzymatic Interactions

Tetrandrine appears to inhibit the P-glycoprotein efflux transporter,[16] with inhibition detectable at 1-2µM in vitro and an oral intake of 10-20mg/kg tetrandrine being able to increase the bioavailability of berberine (normally effluxed by P-glycoprotein);[17] this inhibition of P-glycoprotein also underlies some potential chemotherapeutic roles of tetandrine by overcoming drug resistance.[18][19]

Appears to be a relatively potent and biologically relevant P-glycoprotein inhibitor

Moderate inhibitory potential on CYP3A4 activity with tetrandrine with an IC50 of 3.9µM in vitro.[17]

Tetrandrine has been noted to have no significant inhibitory activity on CYP1A2 (IC50 is somewhere above 100µM) nor CYP2D6 (IC50 is 58.3µM).[17]

The inhibition of CYP3A4 has not been confirmed in vivo, but it seems potent enough to be of concern

3.

Molecular Targets

3.1

Ion Channels

Tetrandrine appears to be a nonselective calcium channel blocker which influences L-type, N-type, and T-type channels; similar in structure and effects as other calcium channel blockers like berbamine (Bereris soulieana) and hernandezine (Thalictrum glandulosissimum).[20][21] It binds to the benzothiazepine/diltiazem recognition site.[22][23][24]

Some sources have also suggested that tetrandrine can inhibit calcium efflux from intracellular stores (calcium channel antagonists tend to inhibit efflux from extracellular compartments to intracellular).[21][25]

3.2

Adrenergic Receptors

Tetrandrine appears to directly interact with α1-adrenergic receptors via competing with prazosin and phenylephrine binding[20] with a Ki value of 690+/-120nM yet IC50 values of 252.8µM (in inhibiting adrenaline induced calcium influx), 11.6µM (inhibiting spontaous basal contractions), and 7.4µM (refilling of calcium stores sensitive to noradrenaline).[26]

Tetrandrine appears to be able to displace ligands of the α1-adrenergic receptors at the prazosin binding site, and moderately to weakly inhibits calcium influx via this receptor

4.

Neurology

4.1

Glutaminergic Neurotransmission

A water extract of stephania tetrandra was able to inhibit NMDA induced currents by 38.65+/-7.50%, which was less effective than both scutellaria baicalensis (83.45+/-4.34%) and salvia miltiorrhiza (52.97+/-1.78%); as these plants had a magnesium content and the study was conducted in vitro, this may not apply to oral ingestion of this herb.[27]

Requires more research to confirm if there are any actual interactions with glutaminergic neurotransmission

4.2

Neuroinflammation

25-50µM tetrandrine appears to attenute LPS-induced microglial activation, reaching around 35% inhibition at 50µM (assessed by superoxide and nitric oxide formation) and appeared to be traced back to NF-kB inhibition.[28]

4.3

Neurprotection

In response to strokes, cells around the infarct area tend to undergo endoplasmic reticulum stress resulting in their apoptosis (cell death).[29][30]

An infusion of 30mg/kg tetrandrine (immediately and 2 hours after ischemia) appears to slightly attenuate cognitive impairment from stroke alongside reducing infarct size and brain edema.[1] While the mechanism was not ascertained, it appeared that the ischemia-induced changes of three proteins (GRP78, DJ-1 and HYOU1) was significantly attenuated, which the authors thought reflected less endoplasmic reticulum stress.[1]

5.

Cardiovascular Health

5.1

Cardiac Tissue

Tetrandrine's main mechanism of action, blocking calcium channels nonspecifically, appears to extend to cardiac tissue where it exerts antiarrythmic effects[14] and has shown anti-arrythmic effects in response to cesium in rabbits[31] as well as ischemia/reperfusion.[32][33]

Tetrandrine appears to reversibly block sodium (Na+) channels in the myocardium in the 60-120μM range by 28.1-87.5% and an IC50 of 74.4-76.9μM.[34]

May possess anti-arrythmic properties on heart tissue via inhibiting ion channels

6.

Interactions with Glucose Metabolism

6.1

Mechanisms

When incubated with HepG2 cells, tetrandrine fails to increase glucose consumption in vitro and failed to interact with both the insulin receptor (expression) and AMPK.[17]

6.2

Blood Glucose

In Streptozotocin induced diabetic mice, the water extract of stephania tetrandra appears to reduce blood glucose in a dose-dependent manner in the range of 0.48—16mg/kg, which is due to fangchinoline as tetrandrine was ineffective.[15] The reduction in blood glucose reached 52.7+/-6.7% with 3mg/kg fangchinoline and was attributed to increased insulin release[15] and this increase in insulin secretion is augmented with calycosin and formononectin form astragalus membranaceus (wherein the combination of 300µg/kg fangchinoline, normally too low a dose, paired with either 30-100µg/kg calycosin or formononectin becomes effective despite the latter two flavonoids not being effective on their own).[35]

Fangchinoline appears to be an effective insulin secretagogue (causes insulin release) in diabetic rats at very low oral doses, and this is synergistic with flavonoids from astragalus membranaceus (a traditional pairing for glucose control)

6.3

Diabetes

Tetrandrine at an oral dose of 20mg/kg daily in rats who normally spontaneously develop diabetes (BB rats) was able to reduce the cumulative occurrence from 75.5 to 10.9% associated with less pancreatic inflammation[36] and FK506 (an immunosuppressive agent that also appears to reduce spontaneous diabetes risk in this same rat model[37]) given for five days alongside tetrandrine appears to be synergistic as delayed administration of tetrandrine reduced diabetes occurrence from 73.1 to 41.7%, whereas the combination with FK506 reduced it to 3.6%.[38]

7.

Skeleton and Bone Metabolism

7.1

Osteoclasts

In osteoclasts, a signalling molecule produced by osteoblasts called 'receptor activator of NF-kB ligand' (RANKL) binds to its receptor (RANK) which then mediates proliferation of osteoclasts[39] via downstream proteins such as MAPKs (ERK and JNK),[40] AP-1,[41] as well as NF-kB itself.[42] An important mediator may be nuclear factor of activated T cells c1 (NFATc1), which can induce the effects associated with RANKL independent of RANKL itself[43] (suggesting that RANKL works via NFATc1).

Tetrandrine at 300-1,000nM is able to inhibit the RANKL-induced activation of NFATc1; specifically, while there was minimal suppressive effect initially the autoamplification of NFATc1 was halved.[44]

Although the exact mechanisms are not known, tetrandrine appears to dysregulate the ability of NFATc1 to autoamplify, which ultimately causes less osteoclastogenesis over the long run

In sciatic-neurectomized mice, injections of 600-2,000µg/kg tetrandrine every other day for four weeks prevented the losses in bone mineral density and trabecular volume, insofar that trabecular bone density in the 600µg/kg group (despite neurectomy) was increased relative to non-neurectomized control.[44]

Injections of very low dose tetrandrine appear to be highly protective of bone mass, insofar to abolish losses in mice subject to neurectomy

8.

Inflammation and Immunology

8.1

Macrophages

Tetrandrine appears to cause macrophage toxicity in vitro (J774 macrophages) with an IC50 value of 50µM, somewhat higher than other mechanisms.[45]

8.2

Neutrophils

Tetrandrine is able to inhibit Mac-1 upregulation at a concentration of 0.1–10μM,[46] Mac-1 which promotes neutrophil adhesion[47] and itself is stimulated by oxidation[48] and calcium ions;[49] tetrandrine suppreses Mac-1 activation secondary to reducing calcium influx[46] although its known antioxidant property[50] also seems to contribute somewhat. The overall anti-adhesive effect is concentration dependent, and can reduce fMLP and PMA induced adhesion to around 60% of baseline at 10µM.[46]

At quite low concentrations, tetrandrine is able to prevent neutrophil adhesion via suppressing both calcium influx and oxidation products within neutrophils

8.3

T Cells

Tetrandrine appears to downregulate PKC dependent signalling in T lymphocytes, and shows suppressive effects on both acute phase T cell activation (calcium influx and IL-2 secretion) at 2-5µM as well as late phase stimulation (CD71 antigen production).[2] This was thought to be independent of calcium signalling since CD40 was unaffected (and CD40 interacts with calcium but not PKC).[2]

8.4

Rheumatism

Traditionally, stephania tetrandra appears to be used against autoimmune diseases such as rheumatism and lupus.[3]

12 weeks supplementation of a water extract of stephania ternatea thrice daily (10g total daily) to persons with diagnosed rheumatoid arthritis was able to reduce human granulocyte elastase (pathogenic factor in some autoimmune diseases[51]) in serum by 56%;[52] actual symptoms of rheumatism were not measured.

8.5

Virology

Fangchinoline appears to inhibit HIV1 replication against multiple strains (NL4-3, LAI, BaL, PM1; inactive in TZM-b1) with an IC50 in the range of 0.8-1.7µM.[53] It appears to target late stages of the infection cycle, and appears to interfere with gp160 proteolytic processing,[53] gp160 being the inactive polypeptide precursor for the envelope proteins that allow HIV replication.[54][55]

At least in vitro, fangchinoline appears to be relatively potent in suppressing HIV1 replication

8.6

Bacteria

Tetrandrine appears to have antibacterial properties against Staphylococcus aureus with a minimal inhibitory concentration (MIC) of 250μg/mL, with an MIC of 125μg/mL against the strains 3102 and 3091 specifically[56] and a similar potency against methicillin resistant strains of Staphylococcus aureus (MRSA).[57]

It appears that tetrandrine can bind directly to peptidoglycan[56] and MRSA tetrandrine appears to be synergistically anti-bacterial with ethidium bromide, reducing the 125-250μg/mL MIC to 31.2-125μg/mL (2 to 4-fold reduction).[57] Elsewhere against strains of candida albicans, tetrandrine appears to be synergistically anti-bacterial when used alongside ketoconazole.[58]

Appears to have some anti-bacterial properties itself (although not overly potent), yet is somewhat synergistic with other pharmacological anti-bacterial agents

9.

Interactions with Oxidation

9.1

In vitro

Tetrandrine appears to be capable of scavenging superoxide radicals in vitro in a concentration dependent manner up to 30µM.[50]

10.

Interactions with Organs

10.1

Kidneys

In isolated rat primary mesangial cells (kidney cells) stimulated with IL-1β (to induce glomerulonephritis in vitro[59]) isolated tetrandrine was able to inhibit the activation of ERK/NF-kB as well as subsequent secretion of MMP9,[60] which are known to correlate with and encourage renal pathology.[61] Tetrandrine was active in the concentration range of 2-10μg/mL, and was more effective (near complete suppression) in regards to iNOS, ERK, and IKK activity but less potent in suppressing IkBα.[60]

Appears to reduce the effects of inflammation upon isolated kidney cells, which appears to occur at a low enough concentration that it may apply to oral supplementation of this herb

10.2

Liver

In rats subject to bile duct ligation, tetrandrine (1-5mg/kg) appears to show anti-fibrotic effects associated with less gene expression (TGF-β, α-SMA, collagen 1α2 and angiogenesis gene products) which was attributed to its NF-kB inhibition.[62] Stephania tetradra (200mg/kg water extract) itself has shown anti-fibrotic potential as assessed by histology (fibrotic area) and stellate cell activation, again attributed to NF-kB inhibition.[63]

When compared to other agents, 5mg/kg tetrandrine is equally effective at reducing collagen accumulation as 50mg/kg silymarins (from milk thistle)[62] and 200mg/kg of the water extract of stephania tetrandra five times weekly for five weeks was as effective as 300mg/kg Salvia miltiorrhiza (water extract) in reducing liver enzymes but more effective in reducing fibrosis from CCl4;[63] pairing stephania tetrandra and salvia miltiorrhiza was not additive in efficacy.[63]

Stephania tetrandra appears to have anti-fibrotic potential that has a comparable or greater potency than other anti-fibrotic supplements

10.3

Penis

In the isolated rabbit corpus cavernosum, tetrandine appears to concentration-dependently (between 100nM and 100µM) increase cAMP concentrations and was able to augment PGE1-induced cAMP accumulation.[64] The authors thought this may reflect PDE enzyme inhibition, but this was not confirmed.[64] Previously, tetrandrine has been noted to inhibit calcium influx into penile tissue[65] and this is known to occur with increases in cAMP concentrations.[66]

Tetrandrine failed to have any influence on cGMP concentrations in the rabbit corpus cavernosum inherently and failed to augment SNP-induced increases in cGMP.[64]

Possibly related to the aforementioned increase in cAMP, tetrandine is known to cause relaxation in precontracted corpus cavernosum penile tissue.[67][68]

May have pro-erectile properties associated with increasing cAMP concentrations, and while the concentration that maximal effects occur is likely too high there may be some benefits (since the lowest active concentration is feasible); no studies using oral ingestion currently exist

11.

Nutrient-Nutrient Interactions

11.1

Astragalus Membranaceus

Astragalus membranaceus appears to be synergistic with stephania tetrandra in reducing blood glucose, as inactive levels of astagalus (3-100mg/kg) and isolated fangchinoline (0.3mg/kg) appear to be active when ingested together in diabetic mice, insofar that 0.3mg/kg fangchinoline in the presence of 30mg/kg astragalus was as effective as 1mg/kg fangchinoline.[15] This appears to be due to the flavonoids known as formononetin and calycosin from astragalus, as these isolated flavonoids at 0.03-0.1mg/kg oral ingestion potentiated 0.3mg/kg fangchinoline.[35]

Astragalus may be synergistic with stephania tetrandra in regards to reducing blood glucose, thought to be secondary to promoting insulin secretion from the pancreas. The oral doses the isolated bioactives are effective is remarkably low, showing a good deal of promise

11.2

Berberine

Berberine is an anti-diabetic alkaloid that works via activating the protein known as AMPK.[69] It is effluxed from the body into the intestines via P-glycoprotein transporters (reducing bioavailability), and the inhibition of P-glycoprotein by tetrandrine increases berberine bioavailability both in vitro[70] and in vivo.[17] Berberine appears to have intestinal efflux (Caco-2) pretty much abolished in vitro with 1µM tetrandrine whereas it was only slightly hindered in liver (HL-7702) cells and not influence in muscle (C2C12) cells which do not express P-glycoprotein.[70]

Furthermore, it appears that the upregulation of P-glycoprotein that berberine exhibits[71][72] is effectively overcome with tetrandrine.[17][70]

Tetrandrine inhibits the transporter that effluxes berberine into the intestines, and by inhibiting this transporter it increases berberine absorption. Tetrandrine may also prevent berberine from upregulating (creatine more) of these transporters

When looking at pharmacokinetic data, 10-20mg/kg tetrandrine alongside 100mg/kg berberine in rats was able to increase the Cmax of berberine in plasma in a dose-dependent manner by 36-62% (reaching 15.4ng/mL and 18.3ng/mL, from 11.3ng/mL in control) and the overall AUC increased to a similar 33-61%.[17] Three weeks of treatment noted that the decreases of blood glucose seen in diabetic rats with 100mg/kg berberine (20.6%) was significantly increased with coingestion of 10mg/kg tetrandrine (55.2%) which even exceeded a double dose of berberine at 200mg/kg (39.5%).[17]

Tetrandrine is able to increase the glucose lowering and anti-diabetic properties of berberine, insofar that adding 1/10th the dose of tetrandrine to berberine is more effective than doubling the berberine dose

12.

Safety and Toxicology

12.1

Case Studies

There are a series of case studies (end stage renal disease) of which causation was placed upon a weight loss supplement said to include stephania tetrandra which was instead cut with another plant bearing the name of Fang Chi, Aristolochia fangchi (or Birthwort);[73] this latter plant contains high levels of the nephrotoxin aristolochic acid.[74][75]

The plant Aristolochia fangchi is one of the four plants, alongside Stephania tetrandra, that is referred to as 'Fang Chi'. It also appears to have a relatively potent kidney toxin in it, and thus supplements that contain aristolochia fangchi rather than stephania tetrandra may cause significant kidney damage

References
2.^Ho LJ, Chang DM, Lee TC, Chang ML, Lai JHPlant alkaloid tetrandrine downregulates protein kinase C-dependent signaling pathway in T cellsEur J Pharmacol.(1999 Feb 19)
13.^Si D, Zhong D, Sha Y, Li WBiflavonoids from the aerial part of Stephania tetrandraPhytochemistry.(2001 Oct)
16.^Fu L, Liang Y, Deng L, Ding Y, Chen L, Ye Y, Yang X, Pan QCharacterization of tetrandrine, a potent inhibitor of P-glycoprotein-mediated multidrug resistanceCancer Chemother Pharmacol.(2004 Apr)
17.^Shan YQ, Zhu YP, Pang J, Wang YX, Song DQ, Kong WJ, Jiang JDTetrandrine potentiates the hypoglycemic efficacy of berberine by inhibiting P-glycoprotein functionBiol Pharm Bull.(2013 Aug 8)
23.^Takemura H, Imoto K, Ohshika H, Kwan CYTetrandrine as a calcium antagonistClin Exp Pharmacol Physiol.(1996 Aug)
24.^Felix JP, King VF, Shevell JL, Garcia ML, Kaczorowski GJ, Bick IR, Slaughter RSBis(benzylisoquinoline) analogs of tetrandrine block L-type calcium channels: evidence for interaction at the diltiazem-binding siteBiochemistry.(1992 Dec 1)
26.^Catret M, Anselmi E, Ivorra MD, Elorriaga M, Tur R, D'Ocón MPAlpha-adrenoceptor interaction of tetrandrine and isotetrandrine in the rat: functional and binding assaysJ Pharm Pharmacol.(1998 Nov)
27.^Sun X, Chan LN, Gong X, Sucher NJN-methyl-D-aspartate receptor antagonist activity in traditional Chinese stroke medicinesNeurosignals.(2003 Jan-Feb)
28.^Xue Y, Wang Y, Feng DC, Xiao BG, Xu LYTetrandrine suppresses lipopolysaccharide-induced microglial activation by inhibiting NF-kappaB pathwayActa Pharmacol Sin.(2008 Feb)
29.^Duan SR, Wang JX, Wang J, Xu R, Zhao JK, Wang DSIschemia induces endoplasmic reticulum stress and cell apoptosis in human brainNeurosci Lett.(2010 May 21)
32.^Yu XC, Wu S, Chen CF, Pang KT, Wong TMAntihypertensive and anti-arrhythmic effects of an extract of Radix Stephaniae Tetrandrae in the ratJ Pharm Pharmacol.(2004 Jan)
34.^Chen L, Li QY, Yang Y, Li ZW, Zeng XRInhibitory effects of tetrandrine on the Na(+) channel of human atrial fibrillation myocardiumActa Pharmacol Sin.(2009 Feb)
36.^Lieberman I, Lentz DP, Trucco GA, Seow WK, Thong YHPrevention by tetrandrine of spontaneous development of diabetes mellitus in BB ratsDiabetes.(1992 May)
37.^Wakugami T, Islam MR, Higa S, Murakami K, Mimura GEffect of FK506 on the development of diabetes in BB rats in comparison with that of cyclosporinTohoku J Exp Med.(1993 Jan)
38.^Lieberman I, Heil BV, Seow WK, Thong YHSynergy between tetrandrine and FK506 in prevention of diabetes in BB ratsLife Sci.(1993)
41.^Grigoriadis AE, Wang ZQ, Cecchini MG, Hofstetter W, Felix R, Fleisch HA, Wagner EFc-Fos: a key regulator of osteoclast-macrophage lineage determination and bone remodelingScience.(1994 Oct 21)
43.^Matsuo K, Galson DL, Zhao C, Peng L, Laplace C, Wang KZ, Bachler MA, Amano H, Aburatani H, Ishikawa H, Wagner EFNuclear factor of activated T-cells (NFAT) rescues osteoclastogenesis in precursors lacking c-FosJ Biol Chem.(2004 Jun 18)
51.^Siedle B, Cisielski S, Murillo R, Löser B, Castro V, Klaas CA, Hucke O, Labahn A, Melzig MF, Merfort ISesquiterpene lactones as inhibitors of human neutrophil elastaseBioorg Med Chem.(2002 Sep)
52.^Sekiya N, Shimada Y, Niizawa A, Kogure T, Mantani N, Sakai S, Hikiami H, Terasawa KSuppressive effects of Stephania tetrandra on the neutrophil function in patients with rheumatoid arthritisPhytother Res.(2004 Mar)
54.^Checkley MA, Luttge BG, Freed EOHIV-1 envelope glycoprotein biosynthesis, trafficking, and incorporationJ Mol Biol.(2011 Jul 22)
55.^Moulard M, Decroly EMaturation of HIV envelope glycoprotein precursors by cellular endoproteasesBiochim Biophys Acta.(2000 Nov 10)
56.^Lee YS, Han SH, Lee SH, Kim YG, Park CB, Kang OH, Keum JH, Kim SB, Mun SH, Seo YS, Myung NY, Kwon DYThe mechanism of antibacterial activity of tetrandrine against Staphylococcus aureusFoodborne Pathog Dis.(2012 Aug)
57.^Lee YS, Han SH, Lee SH, Kim YG, Park CB, Kang OH, Keum JH, Kim SB, Mun SH, Shin DW, Kwon DYSynergistic effect of tetrandrine and ethidium bromide against methicillin-resistant Staphylococcus aureus (MRSA)J Toxicol Sci.(2011 Oct)
58.^Zhang H, Wang K, Zhang G, Ho HI, Gao ASynergistic anti-candidal activity of tetrandrine on ketoconazole: an experimental studyPlanta Med.(2010 Jan)
60.^Wu CJ, Wang YH, Lin CJ, Chen HH, Chen YJTetrandrine down-regulates ERK/NF-κB signaling and inhibits activation of mesangial cellsToxicol In Vitro.(2011 Dec)
61.^Masaki T, Stambe C, Hill PA, Dowling J, Atkins RC, Nikolic-Paterson DJActivation of the extracellular-signal regulated protein kinase pathway in human glomerulopathiesJ Am Soc Nephrol.(2004 Jul)
62.^Hsu YC, Chiu YT, Lee CY, Wu CF, Huang YTAnti-fibrotic effects of tetrandrine on bile-duct ligated ratsCan J Physiol Pharmacol.(2006 Oct)
63.^Chor JS, Yu J, Chan KK, Go YY, Sung JJStephania tetrandra prevents and regresses liver fibrosis induced by carbon tetrachloride in ratsJ Gastroenterol Hepatol.(2009 May)
64.^Chen J, Liu J, Wang T, Xiao H, Yin CEffects of tetrandrine on cAMP and cGMP levels in rabbit corpus cavernosum in vitroNat Prod Res.(2010 Jul)
65.^Liu JH, Chen J, Wang T, Liu B, Yang J, Chen XW, Wang SG, Yin CP, Ye ZQEffects of tetrandrine on cytosolic free calcium concentration in corpus cavernosum smooth muscle cells of rabbitsAsian J Androl.(2006 Jul)
66.^Liu J, Chen JIon channels and penile erectionZhonghua Nan Ke Xue.(2004 Jun)
67.^Chen J, Liu JH, Wang T, Wang SG, Yin CP, Xiao HJ, Liu B, Yang J, Chen XW, Ye ZQThe relaxation effects of six extracts from Chinese herbs on the corpus cavernosum tissue of rabbit in vitroZhonghua Nan Ke Xue.(2005 Oct)
68.^Chen J, Liu JH, Xiao HJ, Wang T, Chen Z, Sun CLThe relaxation effects of tetrandrine on the corpus cavernosum tissue of rabbit in vitroZhonghua Nan Ke Xue.(2003 Jun)
69.^Lee YS, Kim WS, Kim KH, Yoon MJ, Cho HJ, Shen Y, Ye JM, Lee CH, Oh WK, Kim CT, Hohnen-Behrens C, Gosby A, Kraegen EW, James DE, Kim JBBerberine, a natural plant product, activates AMP-activated protein kinase with beneficial metabolic effects in diabetic and insulin-resistant statesDiabetes.(2006 Aug)
70.^Shan YQ, Ren G, Wang YX, Pang J, Zhao ZY, Yao J, You XF, Si SY, Song DQ, Kong WJ, Jiang JDBerberine analogue IMB-Y53 improves glucose-lowering efficacy by averting cellular efflux especially P-glycoprotein effluxMetabolism.(2013 Mar)
71.^Maeng HJ, Yoo HJ, Kim IW, Song IS, Chung SJ, Shim CKP-glycoprotein-mediated transport of berberine across Caco-2 cell monolayersJ Pharm Sci.(2002 Dec)