Damiana Leaf

Last Updated: September 28 2022

Damiana Leaf is a part of the Tunera Diffusa plant which is traditionally used as an aphrodisiac and physical tonic. Not much evidence on this plant, but may be slightly effective in rat models of aphrodisia when coupled with fatigue.

Damiana Leaf is most often used for




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    1.

    Sources and Structure

    1.1

    Sources

    Damiana Leaf is the common name of the plant Turnera diffusa Wild of the Turneraceae family. It possesses a history of aphrodisiac usage (usually brewed as a beverage[1]) where it is known to grow, in Central and Southern America. Although diffusa is the species, this herb is sometimes called Turnera aphrodisiaca due to its reported aphrodisiac properties.[2] Other properties attributed to the plant include stimulant and diuretic properties, as well as being a laxative and kidney tonic.[2]

    1.2

    Composition

    Damiana leaves tend to contain:

    • Acacetin and 7-methyl-acacetin[3]
    • Velutin[3]
    • Turneradiffusin, turneradin and diffusavone[4]
    • Caryophyllene epoxide (with oxide and caryophyllene itself being aromatic oils)[5]
    • Echinacin and echinaticin as well as Z-isomers thereof,[3][6] which are apigenin (7-O-β-d-glucoside) flavonoids with a coumaroyl group attached to the glycoside
    • Pinocembrin[3]
    • Teuhetenone A[3] and Tetraphyllin B[7]
    • 11-hydroxyeremophil-6,9-dien-8-one[3]
    • P-Arbutin,[3] also known as Hydroquinone-β-d-glucopyranoside (both aqueous and methanolic extracts)[8]
    • Damianine (aqueous extract)[8]
    • Gonzalitozin (aqueous extract),[8] a flavonoid[9]
    • Caffeine (aqueous extract)[8]
    • 2″-O-rhamnosylorientin and 2″-O-rhamnosylvitexin[3][6]
    • Apigenin, Chrysoeriol, and Tricin (7-O-β-d-glucoside),[3] the latter two being variants of Apigenin with a methoxy group. Apigenin is present at 0.2-0.24% dry weight of the herb, in the flower and leaves but not fruits or stems[2] Quercetin has also been isolated as a diglycoside[6]
    • Syringetin and Laricitin (3-O-{β-d-glucopyranosyl-(1→6)-β-d-glucopyranoside})[3][6]
    • Luteolin (three glycosides)[3][6]
    • 1,8-cineol as aromatic compound[5] and Delta-Cadinine[5] with all essential oils totalling 0.44% Damiana leaf by weight[10]
    • Beta-Sitosterol[9]
    • Squalene[6]

    image

    Binding a P-E-Coumaroyl group to R1 above results in Echinacin while binding it to R2 results in Echinaticin, the Z-isomers are a result of when a P-Z-Coumaroyl group binds to either of these carbons, respectively.[3]

    2.

    Pharmacology

    2.1

    Enzymatic Interactions

    Damiana Leaf (methanolic extract) was found to, in vitro, inhibit the aromatase enzyme with an IC50 value of 63.1mcg/mL.[3] Compounds from Damiana were tested in isolation, and both pinocembrin and acacetin appeared to be effective in inhibiting 50.5% and 42.6% of aromatase activity at 10μM concentration and having IC50 values of 10.8μM and 18.7μM, respectively.[3] These results were weaker than the active control of aminoglutethimide (5.4+/-0.3μM).[3]

    Moderate efficacy in inhibiting the aromatase enzyme

    3.

    Neurology

    3.1

    Aphrodisia

    In a model of sexually exhausted rats (after 24 hours of ad libitum copulation), Damiana Leaf at 80mg/kg was able to improve mounting, ejaculations, and intromission in male rats with both 20 and 40mg/kg trending towards improvement but failing to be statistically significant.[8] The effects of Damiana were most pronounced during recovering copulation (shortly after copulation), and compared to the active control of Yohimbine at 2mg/kg bodyweight Damiana at 80mg/kg was equally effective at aphrodisia but with a mixed comparison on their pro-erectile effects.[8] These aphrodisiac effects have only been noted once elsewhere, with some benefit to sexually sluggish rats.[11]

    Lacklustre evidence for the aphrodisiac properties of Damiana Leaf, despite historical usage

    3.2

    Anxiety

    When tested in rats using an elevated maze-plus, only the methanolic extract of Damiana Leaf appears to be effective as an anxiolytic at 25mg/kg.[12] This is thought to be due to the Apigenin content.[13] When increasing the dose 12-fold above the effective anxiolytic dose, Damiana appears to have sedative properties.[14]

    Some anxiolytic effects, possibly related to the Apigenin-related molecules, of unknown practical relevance

    3.3

    Analgesia

    In a pain test in rats (tail-flick test), 10mg/kg Apigenin (one of the main bioactives of Damiana) appears to be approximately as effective as 5mg/kg Morphine Sulfate; the pain reduction was dose-dependent.[14] Given a 0.2% Apigenin content of Damiana, this correlates to quite a high oral dose (5g/kg, toxicology untested).

    The bioactive appears to be a very effective pain killer, but the amount of Damiana that must be consumed to reach this level may be excessive and impractical

    4.

    Interactions with Hormones

    4.1

    Estrogen

    Damiana Leaf (methanolic extract) appears to be a weak phytoestrogen, acting on the estrogen receptor with 9% efficacy at 250mcg/mL; this was attributed to the compounds apigenin (7-O-β-d-glucoside), Z-echinacin and pinocembrin with IC50 values of 10, 20, and 67uM respectively.[3]

    5.

    Interactions with Organ Systems

    5.1

    Stomach

    Damiana has been implicated in reducing gastric emptying rate, but the study in question used YGD capsules (Yerba Mate, Guarana, Damiana) and causation cannot be placed on Damiana.[15] YGD capsules have been associated with weight loss due to increased satiety.[16]

    Arbutin isolated from Damiana Leaf at the dose of 30 or 60mg/kg bodyweight for 14 days resulted in gastroprotective effects against an aspirin or an alcohol-induced induction of ulcers in mice, and Arbutin was associated with significantly less lipid peroxidation (MDA) and nitric oxide than both control and active control of omeprazole. Omeprazole (undisclosed concentration) was more effective at reducing the Ulcer Index when administered acutely.[17]

    5.2

    Liver

    In a study on CCL4-induced liver toxicity, Damiana leaves failed to exert significant protective effects in a Huh7 cell line.[18]

    5.3

    Penis

    One study assessing a variety of plants and their ability to induce relaxation on phenylephrine-induced corpus cavernosus contraction (a test for pro-erectile effects) noted inhibition of contraction in the range of 84-95% at 10mg/mL, which was deemed comparable to Damiana Leaf (although Damiana Leaf was not tested). these were more effective than the Viagra used as an active control, but the concentration of Viagra used was low (35mcg/mL).[19]

    6.

    Safety and Toxicology

    Isolated arbutin from Damiana Leaf at up to 2000mg/kg in rats for 2 weeks failed to show any signs of toxicity.[17]

    References
    1.^Lowry TPDamianaJ Psychoactive Drugs.(1984 Jul-Sep)
    2.^Kumar S, Madaan R, Sharma AEstimation of Apigenin, an Anxiolytic Constituent, in Turnera aphrodisiacaIndian J Pharm Sci.(2008 Nov)
    3.^Zhao J, Dasmahapatra AK, Khan SI, Khan IAAnti-aromatase activity of the constituents from damiana (Turnera diffusa)J Ethnopharmacol.(2008 Dec 8)
    5.^Alcaraz-Meléndez L, Delgado-Rodríguez J, Real-Cosío SAnalysis of essential oils from wild and micropropagated plants of damiana (Turnera diffusa)Fitoterapia.(2004 Dec)
    6.^Zhao J, Pawar RS, Ali Z, Khan IAPhytochemical investigation of Turnera diffusaJ Nat Prod.(2007 Feb)
    7.^Spencer KC, Seigler DSTetraphyllin B from Turnera diffusaPlanta Med.(1981 Oct)
    8.^Estrada-Reyes R, Ortiz-López P, Gutiérrez-Ortíz J, Martínez-Mota LTurnera diffusa Wild (Turneraceae) recovers sexual behavior in sexually exhausted malesJ Ethnopharmacol.(2009 Jun 25)
    10.^Kumar S, Taneja R, Sharma APharmacognostic standardization of Turnera aphrodisiaca WardJ Med Food.(2006 Summer)
    11.^Arletti R, Benelli A, Cavazzuti E, Scarpetta G, Bertolini AStimulating property of Turnera diffusa and Pfaffia paniculata extracts on the sexual-behavior of male ratsPsychopharmacology (Berl).(1999 Mar)
    14.^Kumar S, Madaan R, Sharma APharmacological evaluation of Bioactive Principle of Turnera aphrodisiacaIndian J Pharm Sci.(2008 Nov)
    15.^Hui H, Tang G, Go VLHypoglycemic herbs and their action mechanismsChin Med.(2009 Jun 12)
    17.^Taha MM, Salga MS, Ali HM, Abdulla MA, Abdelwahab SI, Hadi AHGastroprotective activities of Turnera diffusa Willd. ex Schult. revisited: Role of arbutinJ Ethnopharmacol.(2012 May 7)
    18.^Torres-González L, Muñoz-Espinosa LE, Rivas-Estilla AM, Trujillo-Murillo K, Salazar-Aranda R, Waksman De Torres N, Cordero-Pérez PProtective effect of four Mexican plants against CCl₄-induced damage on the Huh7 human hepatoma cell lineAnn Hepatol.(2011 Jan-Mar)
    19.^Hnatyszyn O, Moscatelli V, Garcia J, Rondina R, Costa M, Arranz C, Balaszczuk A, Ferraro G, Coussio JDArgentinian plant extracts with relaxant effect on the smooth muscle of the corpus cavernosum of guinea pigPhytomedicine.(2003 Nov)