Biotin
•Last Updated: September 28 2022
Biotin is one of the essential B vitamins used by the body primarily as an enzymatic cofactor. While it is popular as a beauty supplement for hair, skin, and nails and very preliminary evidence suggests it may have a role in these uses, its role is not well supported. Potential interactions with diabetes are also not well understood.
Biotin is most often used for
Last Updated: September 28 2022
Biotin is an essential vitamin that has been grouped with the B-complex vitamins since it was discovered in yeast alongside other B vitamins. Although it is technically known as vitamin B7, this designation is not too common as it is usually simply referred to as biotin.
It was initially found to be a component of nails, skin, and hair to a relatively high degree. Biotin has been seen as the go-to vitamin for beauty ever since one pilot study in women with brittle nails showed supplementation to be beneficial. It is currently being marketed for improving nail, skin, and hair aesthetics. These claims, however, were not followed up scientifically so there is not much evidence to support biotin's role here. It can plausibly have these actions mechanistically, but there is simply not much evidence that can be used as support.
Beyond that, biotin's general role as an enzymatic cofactor has also led to some research suggesting it may interact with glucose metabolism in the human body. As a general statement, it seems that in rodents with a higher circulating biotin level in their blood, the amount of insulin released in response to a glucose test is higher, leading to less elevation of glucose over time. This rodent evidence also reports that the higher glucose is not met with higher insulin resistance, suggesting a potentially beneficial role.
Not too much evidence has been conducted in humans in regard to diabetes, with one study finding that intramuscular biotin was able to attenuate symptoms of neuropathy in three diabetic subjects.
Overall, aside from instances where biotin may be deficient (alcoholism, some epileptic drug therapies, and overconsumption of raw egg whites) the supplement does not have any solid evidence for benefits and may have a role as a beauty supplement pending better evidence.
Note that Biotin is also known as:
- Vitamin B7
- Vitamin H
The only known supplemental dose of biotin that has been tested orally in humans — for the purposes of enhancing the quality of brittle nails — is 2.5mg taken once daily over six months.
This dose appears relatively safe, although it is much higher than the recommended daily intake (RDI) of biotin which ranges from 25-30mcg (youth) upwards to 100mcg (adults). The biotin dose found in many multivitamins (30mcg or 0.03mg) seems more than sufficient.
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1.^Chapman-Smith A1, Cronan JE JrMolecular biology of biotin attachment to proteinsJ Nutr.(1999 Feb)
3.^Chapman-Smith A1, Cronan JE JrThe enzymatic biotinylation of proteins: a post-translational modification of exceptional specificityTrends Biochem Sci.(1999 Sep)
6.^Tong L1, Harwood HJ JrAcetyl-coenzyme A carboxylases: versatile targets for drug discoveryJ Cell Biochem.(2006 Dec 15)
7.^Huang CS1, Sadre-Bazzaz K, Shen Y, Deng B, Zhou ZH, Tong LCrystal structure of the alpha(6)beta(6) holoenzyme of propionyl-coenzyme A carboxylaseNature.(2010 Aug 19)
8.^Xiang S1, Tong LCrystal structures of human and Staphylococcus aureus pyruvate carboxylase and molecular insights into the carboxyltransfer reactionNat Struct Mol Biol.(2008 Mar)
9.^Depeint F1, Bruce WR, Shangari N, Mehta R, O'Brien PJMitochondrial function and toxicity: role of the B vitamin family on mitochondrial energy metabolismChem Biol Interact.(2006 Oct 27)
12.^Shriver BJ1, Roman-Shriver C, Allred JBDepletion and repletion of biotinyl enzymes in liver of biotin-deficient rats: evidence of a biotin storage systemJ Nutr.(1993 Jun)
13.^Hymes J1, Fleischhauer K, Wolf BBiotinylation of histones by human serum biotinidase: assessment of biotinyl-transferase activity in sera from normal individuals and children with biotinidase deficiencyBiochem Mol Med.(1995 Oct)
14.^Kuroishi T1, Rios-Avila L, Pestinger V, Wijeratne SS, Zempleni JBiotinylation is a natural, albeit rare, modification of human histonesMol Genet Metab.(2011 Dec)
15.^Hymes J, Fleischhauer K, Wolf BBiotinylation of histones by human serum biotinidase: assessment of biotinyl-transferase activity in sera from normal individuals and children with biotinidase deficiencyBiochem Mol Med.(1995 Oct)
16.^Healy S1, Heightman TD, Hohmann L, Schriemer D, Gravel RANonenzymatic biotinylation of histone H2AProtein Sci.(2009 Feb)
17.^Eng WK1, Giraud D, Schlegel VL, Wang D, Lee BH, Zempleni JIdentification and assessment of markers of biotin status in healthy adultsBr J Nutr.(2013 Jul 28)
18.^Mock NI1, Malik MI, Stumbo PJ, Bishop WP, Mock DMIncreased urinary excretion of 3-hydroxyisovaleric acid and decreased urinary excretion of biotin are sensitive early indicators of decreased biotin status in experimental biotin deficiencyAm J Clin Nutr.(1997 Apr)
20.^Mock DM1, Henrich CL, Carnell N, Mock NIIndicators of marginal biotin deficiency and repletion in humans: validation of 3-hydroxyisovaleric acid excretion and a leucine challengeAm J Clin Nutr.(2002 Nov)
21.^Nisenson ASeborrheic dermatitis of infants: treatment with biotin injections for the nursing motherPediatrics.(1969 Dec)
22.^Wolf BBiotinidase deficiency: "if you have to have an inherited metabolic disease, this is the one to have"Genet Med.(2012 Jun)
23.^Cowan TM1, Blitzer MG, Wolf B; Working Group of the American College of Medical Genetics Laboratory Quality Assurance CommitteeTechnical standards and guidelines for the diagnosis of biotinidase deficiencyGenet Med.(2010 Jul)
24.^Wolf BWorldwide survey of neonatal screening for biotinidase deficiencyJ Inherit Metab Dis.(1991)
26.^Sydenstricker VP, Singal SA, Briggs AP, DeVaughn NM, Harris IOBSERVATIONS ON THE "EGG WHITE INJURY" IN MAN AND ITS CURE WITH A BIOTIN CONCENTRATEJAMA.(1942 Apr)
27.^Baugh CM, Malone JH, Butterworth CE JrHuman biotin deficiency. A case history of biotin deficiency induced by raw egg consumption in a cirrhotic patientAm J Clin Nutr.(1968 Feb)
28.^WEI RD, WRIGHT LDHEAT STABILITY OF AVIDIN AND AVIDIN-BIOTIN COMPLEX AND INFLUENCE OF IONIC STRENGTH ON AFFINITY OF AVIDIN FOR BIOTINProc Soc Exp Biol Med.(1964 Nov)
29.^Murthy CV, Adiga PRPurification of biotin-binding protein from chicken egg yolk and comparison with avidinBiochim Biophys Acta.(1984 May 17)
30.^White HB 3rd, Dennison BA, Della Fera MA, Whitney CJ, McGuire JC, Meslar HW, Sammelwitz PHBiotin-binding protein from chicken egg yolk. Assay and relationship to egg-white avidinBiochem J.(1976 Aug 1)
31.^Vesely DL, Kemp SF, Elders MJIsolation of a biotin receptor from hepatic plasma membranesBiochem Biophys Res Commun.(1987 Mar 30)
32.^Leamon CP1, Low PSDelivery of macromolecules into living cells: a method that exploits folate receptor endocytosisProc Natl Acad Sci U S A.(1991 Jul 1)
33.^Wuerges J1, Geremia S, Randaccio LStructural study on ligand specificity of human vitamin B12 transportersBiochem J.(2007 May 1)
34.^Horn MA1, Heinstein PF, Low PSBiotin-mediated delivery of exogenous macromolecules into soybean cellsPlant Physiol.(1990 Aug)
35.^Russell-Jones G1, McTavish K, McEwan J, Rice J, Nowotnik DVitamin-mediated targeting as a potential mechanism to increase drug uptake by tumoursJ Inorg Biochem.(2004 Oct)
36.^Yellepeddi VK1, Kumar A, Palakurthi SBiotinylated poly(amido)amine (PAMAM) dendrimers as carriers for drug delivery to ovarian cancer cells in vitroAnticancer Res.(2009 Aug)
37.^Chauhan J, Dakshinamurti KRole of human serum biotinidase as biotin-binding proteinBiochem J.(1988 Nov 15)
38.^Said HM1, Redha R, Nylander WA carrier-mediated, Na+ gradient-dependent transport for biotin in human intestinal brush-border membrane vesiclesAm J Physiol.(1987 Nov)
39.^Said HM1, Redha RBiotin transport in rat intestinal brush-border membrane vesiclesBiochim Biophys Acta.(1988 Nov 22)
40.^Said HM1, Derweesh ICarrier-mediated mechanism for biotin transport in rabbit intestine: studies with brush-border membrane vesiclesAm J Physiol.(1991 Jul)
41.^Balamurugan K1, Ortiz A, Said HMBiotin uptake by human intestinal and liver epithelial cells: role of the SMVT systemAm J Physiol Gastrointest Liver Physiol.(2003 Jul)
42.^Prasad PD1, Wang H, Kekuda R, Fujita T, Fei YJ, Devoe LD, Leibach FH, Ganapathy VCloning and functional expression of a cDNA encoding a mammalian sodium-dependent vitamin transporter mediating the uptake of pantothenate, biotin, and lipoateJ Biol Chem.(1998 Mar 27)
43.^Subramanian VS1, Marchant JS, Boulware MJ, Ma TY, Said HMMembrane targeting and intracellular trafficking of the human sodium-dependent multivitamin transporter in polarized epithelial cellsAm J Physiol Cell Physiol.(2009 Apr)
44.^Subramanya SB1, Subramanian VS, Kumar JS, Hoiness R, Said HMInhibition of intestinal biotin absorption by chronic alcohol feeding: cellular and molecular mechanismsAm J Physiol Gastrointest Liver Physiol.(2011 Mar)
45.^Branner GR1, Roth-Maier DAInfluence of pre-, pro-, and synbiotics on the intestinal availability of different B-vitaminsArch Anim Nutr.(2006 Jun)
46.^Wang H1, Huang W, Fei YJ, Xia H, Yang-Feng TL, Leibach FH, Devoe LD, Ganapathy V, Prasad PDHuman placental Na+-dependent multivitamin transporter. Cloning, functional expression, gene structure, and chromosomal localizationJ Biol Chem.(1999 May 21)
47.^Said HM1, Horne DW, Mock DMEffect of aging on intestinal biotin transport in the ratExp Gerontol.(1990)
48.^Said HM1, Mock DM, Collins JCRegulation of intestinal biotin transport in the rat: effect of biotin deficiency and supplementationAm J Physiol.(1989 Feb)
49.^León-Del-Río A1, Hol-Soto-Borja D, Velázquez AStudies on the mechanism of biotin uptake by brush-border membrane vesicles of hamster enterocytesArch Med Res.(1993 Summer)
50.^Zempleni J1, Mock DMUptake and metabolism of biotin by human peripheral blood mononuclear cellsAm J Physiol.(1998 Aug)
51.^Grafe F1, Wohlrab W, Neubert RH, Brandsch MTransport of biotin in human keratinocytesJ Invest Dermatol.(2003 Mar)
52.^Makino Y1, Osada K, Sone H, Sugiyama K, Komai M, Ito M, Tsunoda K, Furukawa YPercutaneous absorption of biotin in healthy subjects and in atopic dermatitis patientsJ Nutr Sci Vitaminol (Tokyo).(1999 Jun)
53.^Gilby ED, Taylor KJUltrasound monitoring of hepatic metastases during chemotherapyBr Med J.(1975 Feb 15)
54.^Mock DM1, Lankford GL, Mock NIBiotin accounts for only half of the total avidin-binding substances in human serumJ Nutr.(1995 Apr)
55.^Mock DM1, Lankford GL, Cazin J JrBiotin and biotin analogs in human urine: biotin accounts for only half of the totalJ Nutr.(1993 Nov)
56.^Zempleni J1, McCormick DB, Mock DMIdentification of biotin sulfone, bisnorbiotin methyl ketone, and tetranorbiotin-l-sulfoxide in human urineAm J Clin Nutr.(1997 Feb)
57.^Bogusiewicz A1, Stratton SL, Ellison DA, Mock DMBiotin accounts for less than half of all biotin and biotin metabolites in the cerebrospinal fluid of childrenAm J Clin Nutr.(2008 Nov)
58.^Marshall MW, Kliman PG, Washington VA, Mackin JF, Weinland BTEffects of biotin on lipids and other constituents of plasma of healthy men and womenArtery.(1980)
59.^Beinlich CJ1, Naumovitz RD, Song WO, Neely JRMyocardial metabolism of pantothenic acid in chronically diabetic ratsJ Mol Cell Cardiol.(1990 Mar)
60.^Baur B1, Wick H, Baumgartner ERNa(+)-dependent biotin transport into brush-border membrane vesicles from rat kidneyAm J Physiol.(1990 Apr)
61.^Baur B1, Baumgartner ERBiotin and biocytin uptake into cultured primary calf brain microvessel endothelial cells of the blood-brain barrierBrain Res.(2000 Mar 10)
62.^Said HM1, Korchid S, Horne DW, Howard MTransport of biotin in basolateral membrane vesicles of rat liverAm J Physiol.(1990 Nov)
64.^Prasad PD1, Wang H, Huang W, Fei YJ, Leibach FH, Devoe LD, Ganapathy VMolecular and functional characterization of the intestinal Na+-dependent multivitamin transporterArch Biochem Biophys.(1999 Jun 1)
66.^Castro-Gago M1, Gómez-Lado C, Eirís-Puñal J, Díaz-Mayo I, Castiñeiras-Ramos DESerum biotinidase activity in children treated with valproic acid and carbamazepineJ Child Neurol.(2010 Jan)
67.^Castro-Gago M1, Pérez-Gay L, Gómez-Lado C, Castiñeiras-Ramos DE, Otero-Martínez S, Rodríguez-Segade SThe influence of valproic acid and carbamazepine treatment on serum biotin and zinc levels and on biotinidase activityJ Child Neurol.(2011 Dec)
68.^Suchy SF, Wolf BEffect of biotin deficiency and supplementation on lipid metabolism in rats: cholesterol and lipoproteinsAm J Clin Nutr.(1986 May)
69.^Suchy SF, Wolf BEffect of biotin deficiency and supplementation on lipid metabolism in rats: cholesterol and lipoproteinsAm J Clin Nutr.(1986 May)
70.^Larrieta E1, Velasco F, Vital P, López-Aceves T, Lazo-de-la-Vega-Monroy ML, Rojas A, Fernandez-Mejia CPharmacological concentrations of biotin reduce serum triglycerides and the expression of lipogenic genesEur J Pharmacol.(2010 Oct 10)
71.^Aguilera-Méndez A1, Fernández-Mejía CThe hypotriglyceridemic effect of biotin supplementation involves increased levels of cGMP and AMPK activationBiofactors.(2012 Sep-Oct)
72.^Ha J1, Daniel S, Broyles SS, Kim KHCritical phosphorylation sites for acetyl-CoA carboxylase activityJ Biol Chem.(1994 Sep 2)
73.^Dokusova OK, Krivoruchenko IVThe effect of biotin on the level of cholesterol in the blood of patients with atherosclerosis and essential hyperlipidemiaKardiologiia.(1972 Dec)
74.^Revilla-Monsalve C1, Zendejas-Ruiz I, Islas-Andrade S, Báez-Saldaña A, Palomino-Garibay MA, Hernández-Quiróz PM, Fernandez-Mejia CBiotin supplementation reduces plasma triacylglycerol and VLDL in type 2 diabetic patients and in nondiabetic subjects with hypertriglyceridemiaBiomed Pharmacother.(2006 May)
75.^Sarabu R1, Grimsby JTargeting glucokinase activation for the treatment of type 2 diabetes--a status reviewCurr Opin Drug Discov Devel.(2005 Sep)
76.^Dakshinamurti K, Cheah-Tan CLiver glucokinase of the biotin deficient ratCan J Biochem.(1968 Jan)
78.^Spence JT, Koudelka APEffects of biotin upon the intracellular level of cGMP and the activity of glucokinase in cultured rat hepatocytesJ Biol Chem.(1984 May 25)
79.^Agius LControl of glucokinase translocation in rat hepatocytes by sorbitol and the cytosolic redox stateBiochem J.(1994 Feb 15)
80.^Davagnino J, Ureta TThe identification of extrahepatic "glucokinase" as N-acetylglucosamine kinaseJ Biol Chem.(1980 Apr 10)
81.^Spence JT, Pitot HCInduction of lipogenic enzymes in primary cultures of rat hepatocytes. Relationship between lipogenesis and carbohydrate metabolismEur J Biochem.(1982 Nov)
82.^Chauhan J1, Dakshinamurti KTranscriptional regulation of the glucokinase gene by biotin in starved ratsJ Biol Chem.(1991 Jun 5)
83.^Dakshinamurti K, Cheah-Tan CBiotin-mediated synthesis of hepatic glucokinase in the ratArch Biochem Biophys.(1968 Sep 20)
84.^Vilches-Flores A1, Tovar AR, Marin-Hernandez A, Rojas-Ochoa A, Fernandez-Mejia CBiotin increases glucokinase expression via soluble guanylate cyclase/protein kinase G, adenosine triphosphate production and autocrine action of insulin in pancreatic rat isletsJ Nutr Biochem.(2010 Jul)
85.^De La Vega LA1, Stockert RJRegulation of the insulin and asialoglycoprotein receptors via cGMP-dependent protein kinaseAm J Physiol Cell Physiol.(2000 Dec)
86.^Lazo de la Vega-Monroy ML1, Larrieta E, German MS, Baez-Saldana A, Fernandez-Mejia CEffects of biotin supplementation in the diet on insulin secretion, islet gene expression, glucose homeostasis and beta-cell proportionJ Nutr Biochem.(2013 Jan)
87.^Sasaki Y, Sone H, Kamiyama S, Shimizu M, Shirakawa H, Kagawa Y, Komai M, Furukawa YAdministration of biotin prevents the development of insulin resistance in the skeletal muscles of Otsuka Long-Evans Tokushima Fatty ratsFood Funct.(2012 Apr)
88.^Koutsikos D1, Agroyannis B, Tzanatos-Exarchou HBiotin for diabetic peripheral neuropathyBiomed Pharmacother.(1990)
89.^Krause KH, Berlit P, Bonjour JPImpaired biotin status in anticonvulsant therapyAnn Neurol.(1982 Nov)
90.^Bonjour JPVitamins and alcoholism. V. Riboflavin, VI. Niacin, VII. Pantothenic acid, and VIII. BiotinInt J Vitam Nutr Res.(1980)
91.^THOMPSON RH, BUTTERFIELD WJ, FRY IKPyruvate metabolism in diabetic neuropathyProc R Soc Med.(1960 Feb)
92.^Shriver BJ, Roman-Shriver C, Allred JBDepletion and repletion of biotinyl enzymes in liver of biotin-deficient rats: evidence of a biotin storage systemJ Nutr.(1993 Jun)
93.^Xu C1, Bai B, Fan P, Cai Y, Huang B, Law IK, Liu L, Xu A, Tung C, Li X, Siu FM, Che CM, Vanhoutte PM, Wang YSelective overexpression of human SIRT1 in adipose tissue enhances energy homeostasis and prevents the deterioration of insulin sensitivity with ageing in miceAm J Transl Res.(2013 May 24)
94.^Law IK1, Liu L, Xu A, Lam KS, Vanhoutte PM, Che CM, Leung PT, Wang YIdentification and characterization of proteins interacting with SIRT1 and SIRT3: implications in the anti-aging and metabolic effects of sirtuinsProteomics.(2009 May)
95.^Mao J1, Yang T, Gu Z, Heird WC, Finegold MJ, Lee B, Wakil SJaP2-Cre-mediated inactivation of acetyl-CoA carboxylase 1 causes growth retardation and reduced lipid accumulation in adipose tissuesProc Natl Acad Sci U S A.(2009 Oct 13)
96.^Fukuwatari T1, Wada H, Shibata KAge-related alterations of B-group vitamin contents in urine, blood and liver from ratsJ Nutr Sci Vitaminol (Tokyo).(2008 Oct)
98.^Gschwandtner M1, Mildner M, Mlitz V, Gruber F, Eckhart L, Werfel T, Gutzmer R, Elias PM, Tschachler EHistamine suppresses epidermal keratinocyte differentiation and impairs skin barrier function in a human skin modelAllergy.(2013 Jan)
99.^Ogawa Y1, Kiba T, Nakano K, Fujiwara K, Taniguchi H, Hosokawa A, Nakashima T, Kimoto S, Kajiume S, Okada Y, Ichiba YProspective study of biotin treatment in patients with erythema due to gefitinib or erlotinibGan To Kagaku Ryoho.(2014 Apr)
100.^Zempleni J1, Hassan YI, Wijeratne SSBiotin and biotinidase deficiencyExpert Rev Endocrinol Metab.(2008 Nov 1)
101.^Korkmazer N1, Vurucu S, Demirkaya E, Unay B, Kul M, Akin R, Gokcay ESerum and liver tissue biotinidase enzyme activity in rats which were administrated to valproic acidBrain Dev.(2006 Sep)
102.^Arslan M1, Vurucu S, Balamtekin N, Unay B, Akin R, Kurt I, Ozcan OThe effects of biotin supplementation on serum and liver tissue biotinidase enzyme activity and alopecia in rats which were administrated to valproic acidBrain Dev.(2009 Jun)
103.^Schulpis KH1, Karikas GA, Tjamouranis J, Regoutas S, Tsakiris SLow serum biotinidase activity in children with valproic acid monotherapyEpilepsia.(2001 Oct)
104.^Luís PB1, Ruiter JP, IJlst L, Diogo L, Garcia P, de Almeida IT, Duran M, Wanders RJ, Silva MFInhibition of 3-methylcrotonyl-CoA carboxylase explains the increased excretion of 3-hydroxyisovaleric acid in valproate-treated patientsJ Inherit Metab Dis.(2012 May)
105.^Comben N, Clark RJ, Sutherland DJClinical observations on the response of equine hoof defects to dietary supplementation with biotinVet Rec.(1984 Dec 22-29)
106.^CUNHA TJ, LINDLEY DC, ENSMINGER MEBiotin deficiency syndrome in pigs fed desicated egg whiteJ Anim Sci.(1946 May)
107.^Colombo VE, Gerber F, Bronhofer M, Floersheim GLTreatment of brittle fingernails and onychoschizia with biotin: scanning electron microscopyJ Am Acad Dermatol.(1990 Dec)
108.^Colombo VE1, Gerber F, Bronhofer M, Floersheim GLTreatment of brittle fingernails and onychoschizia with biotin: scanning electron microscopyJ Am Acad Dermatol.(1990 Dec)
109.^Hale G, Wallis NG, Perham RNInteraction of avidin with the lipoyl domains in the pyruvate dehydrogenase multienzyme complex: three-dimensional location and similarity to biotinyl domains in carboxylasesProc Biol Sci.(1992 Jun 22)
110.^Zempleni J, Trusty TA, Mock DMLipoic acid reduces the activities of biotin-dependent carboxylases in rat liverJ Nutr.(1997 Sep)
111.^Zempleni J1, Trusty TA, Mock DMLipoic acid reduces the activities of biotin-dependent carboxylases in rat liverJ Nutr.(1997 Sep)
112.^Said HM1, Sharifian A, Bagherzadeh A, Mock DChronic ethanol feeding and acute ethanol exposure in vitro: effect on intestinal transport of biotinAm J Clin Nutr.(1990 Dec)
113.^FENNELLY J, FRANK O, BAKER H, LEEVY CMPERIPHERAL NEUROPATHY OF THE ALCOHOLIC: I, AETIOLOGICAL ROLE OF ANEURIN AND OTHER B-COMPLEX VITAMINSBr Med J.(1964 Nov 21)
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