Rhamnus Nakaharia (from the family Rhamnaceae) is a folk medicine originating in Taiwan that has been used to treat constipation, inﬂammation, malignant tumors, and asthma.
Rhamnus Nakaharai (RN) is a Taiwanese herbal medicine that contains:
The napthalenic compound 6-methoxysorigenin and various glycosides and acylates of the parent compound, such as alpha-sorinin although 6-methoxyorigenin appears to be isolated from the wood component of the herb
The compound 6-methoxyorigenin appears to have anti-oxidative effects in vitro, as well as the related compound Isotorachrysone, which share structural similarities. 6-methoxygenin outperformed Trolox (a research standard) on Metal-chelating and protecting from lipid peroxidation, and was slightly outperformed on a DPPH test. The IC50 of the metal chelating ability of 6-methoxyorigenin (615.90+/-5.79) was lesser than that of EDTA (127.92+/-6.27).
3-O-Methylquercetin has been found to suppress the inflammatory (Nitric Oxide mediated) response from LPS (research pro-inflammatory agent) in macrophages in vitro. 3-O-Methylquercetin was found to inhibit Nitric Oxide release, protein expression of iNOS (induced by inflammation) and mRNA translation of iNOS with IC50 values of 4.23uM, 4.36uM, and 6.53uM respectively.
3-O-Methylquercetin appears to be a selective PDE3 inhibitor with an IC50 of 1.6mM via competitive inhibition.