Arachidonic Acid

Last Updated: September 28 2022

Arachidonic acid (AA) is a fatty acid of the omega-6 class, and is the main fatty acid of interest when referring to an omega-3:6 ratio (relative to fish oil fatty acids). It is proinflammatory and immunosupportive.

Arachidonic Acid is most often used for

What else is Arachidonic Acid known as?
Note that Arachidonic Acid is also known as:
  • ARA
Arachidonic Acid should not be confused with:
  • Linoleic acid (parent omega-6 fatty acid)
Dosage information

There is currently insufficient evidence to recommend an ideal dose of arachidonic acid supplementation, but anecdotally it is used at the dosage of around 2,000 mg taken 45 minutes before a workout. It is uncertain if this is an optimal dose or whether the timing is required.

It should also be noted that for persons with chronic inflammatory disorders, such as rheumatoid arthritis or inflammatory bowel diseases, that the ideal dose of arachidonic acid may actually be a dietary restriction thereof. In instances of inflammatory diseases, arachidonic acid supplementation is likely contraindicated.

Examine Database
References
1.^Kawabata T, Hirota S, Hirayama T, Adachi N, Hagiwara C, Iwama N, Kamachi K, Araki E, Kawashima H, Kiso YAge-related changes of dietary intake and blood eicosapentaenoic acid, docosahexaenoic acid, and arachidonic acid levels in Japanese men and womenProstaglandins Leukot Essent Fatty Acids.(2011 May-Jun)
2.^Linseisen J, Schulze MB, Saadatian-Elahi M, Kroke A, Miller AB, Boeing HQuantity and quality of dietary fat, carbohydrate, and fiber intake in the German EPIC cohortsAnn Nutr Metab.(2003)
3.^Sioen IA, Pynaert I, Matthys C, De Backer G, Van Camp J, De Henauw SDietary intakes and food sources of fatty acids for Belgian women, focused on n-6 and n-3 polyunsaturated fatty acidsLipids.(2006 May)
4.^Mann NJ, Johnson LG, Warrick GE, Sinclair AJThe arachidonic acid content of the Australian diet is lower than previously estimatedJ Nutr.(1995 Oct)
8.^Li D, Ng A, Mann NJ, Sinclair AJContribution of meat fat to dietary arachidonic acidLipids.(1998 Apr)
9.^Brignoli CA, Kinsella JE, Weihrauch JLComprehensive evaluation of fatty acids in foods. V. Unhydrogenated fats and oilsJ Am Diet Assoc.(1976 Mar)
10.^Anderson BA, Kinsella JA, Watt BKComprehensive evaluation of fatty acids in foods. II. Beef productsJ Am Diet Assoc.(1975 Jul)
14.^James MJ, Gibson RA, D'Angelo M, Neumann MA, Cleland LGSimple relationships exist between dietary linoleate and the n-6 fatty acids of human neutrophils and plasmaAm J Clin Nutr.(1993 Oct)
16.^Whelan J, Surette ME, Hardardóttir I, Lu G, Golemboski KA, Larsen E, Kinsella JEDietary arachidonate enhances tissue arachidonate levels and eicosanoid production in Syrian hamstersJ Nutr.(1993 Dec)
18.^Adam O, Beringer C, Kless T, Lemmen C, Adam A, Wiseman M, Adam P, Klimmek R, Forth WAnti-inflammatory effects of a low arachidonic acid diet and fish oil in patients with rheumatoid arthritisRheumatol Int.(2003 Jan)
19.^Khanna IK, Alexander CWFatty acid amide hydrolase inhibitors--progress and potentialCNS Neurol Disord Drug Targets.(2011 Aug)
21.^Watanabe H, Vriens J, Prenen J, Droogmans G, Voets T, Nilius BAnandamide and arachidonic acid use epoxyeicosatrienoic acids to activate TRPV4 channelsNature.(2003 Jul 24)
22.^Nomura DK, Hudak CS, Ward AM, Burston JJ, Issa RS, Fisher KJ, Abood ME, Wiley JL, Lichtman AH, Casida JEMonoacylglycerol lipase regulates 2-arachidonoylglycerol action and arachidonic acid levelsBioorg Med Chem Lett.(2008 Nov 15)
23.^Ueda NEndocannabinoid hydrolasesProstaglandins Other Lipid Mediat.(2002 Aug)
24.^Söderberg M, Edlund C, Kristensson K, Dallner GFatty acid composition of brain phospholipids in aging and in Alzheimer's diseaseLipids.(1991 Jun)
26.^Maniongui C, Blond JP, Ulmann L, Durand G, Poisson JP, Bézard JAge-related changes in delta 6 and delta 5 desaturase activities in rat liver microsomesLipids.(1993 Apr)
31.^Walev I, Klein J, Husmann M, Valeva A, Strauch S, Wirtz H, Weichel O, Bhakdi SPotassium regulates IL-1 beta processing via calcium-independent phospholipase A2J Immunol.(2000 May 15)
35.^Wada M, DeLong CJ, Hong YH, Rieke CJ, Song I, Sidhu RS, Yuan C, Warnock M, Schmaier AH, Yokoyama C, Smyth EM, Wilson SJ, FitzGerald GA, Garavito RM, Sui de X, Regan JW, Smith WLEnzymes and receptors of prostaglandin pathways with arachidonic acid-derived versus eicosapentaenoic acid-derived substrates and productsJ Biol Chem.(2007 Aug 3)
36.^Patrono CAspirin as an antiplatelet drugN Engl J Med.(1994 May 5)
38.^Chan CC, Boyce S, Brideau C, Ford-Hutchinson AW, Gordon R, Guay D, Hill RG, Li CS, Mancini J, Penneton M, et alPharmacology of a selective cyclooxygenase-2 inhibitor, L-745,337: a novel nonsteroidal anti-inflammatory agent with an ulcerogenic sparing effect in rat and nonhuman primate stomachJ Pharmacol Exp Ther.(1995 Sep)
40.^Evett GE, Xie W, Chipman JG, Robertson DL, Simmons DLProstaglandin G/H synthase isoenzyme 2 expression in fibroblasts: regulation by dexamethasone, mitogens, and oncogenesArch Biochem Biophys.(1993 Oct)
42.^Yamagata K, Andreasson KI, Kaufmann WE, Barnes CA, Worley PFExpression of a mitogen-inducible cyclooxygenase in brain neurons: regulation by synaptic activity and glucocorticoidsNeuron.(1993 Aug)
46.^Urade Y, Hayaishi OProstaglandin D synthase: structure and functionVitam Horm.(2000)
47.^Pettipher R, Hansel TTAntagonists of the prostaglandin D2 receptor CRTH2Drug News Perspect.(2008 Jul-Aug)
48.^Nagata K, Tanaka K, Ogawa K, Kemmotsu K, Imai T, Yoshie O, Abe H, Tada K, Nakamura M, Sugamura K, Takano SSelective expression of a novel surface molecule by human Th2 cells in vivoJ Immunol.(1999 Feb 1)
49.^Garza LA, Liu Y, Yang Z, Alagesan B, Lawson JA, Norberg SM, Loy DE, Zhao T, Blatt HB, Stanton DC, Carrasco L, Ahluwalia G, Fischer SM, FitzGerald GA, Cotsarelis GProstaglandin D2 inhibits hair growth and is elevated in bald scalp of men with androgenetic alopeciaSci Transl Med.(2012 Mar 21)
52.^Hardy CC, Robinson C, Tattersfield AE, Holgate STThe bronchoconstrictor effect of inhaled prostaglandin D2 in normal and asthmatic menN Engl J Med.(1984 Jul 26)
53.^Beasley CR, Robinson C, Featherstone RL, Varley JG, Hardy CC, Church MK, Holgate ST9 alpha,11 beta-prostaglandin F2, a novel metabolite of prostaglandin D2 is a potent contractile agonist of human and guinea pig airwaysJ Clin Invest.(1987 Mar)
54.^Bochenek G, Nizankowska E, Gielicz A, Swierczyńska M, Szczeklik APlasma 9alpha,11beta-PGF2, a PGD2 metabolite, as a sensitive marker of mast cell activation by allergen in bronchial asthmaThorax.(2004 Jun)
55.^Kawabata AProstaglandin E2 and pain--an updateBiol Pharm Bull.(2011)
56.^Mbalaviele G, Pauley AM, Shaffer AF, Zweifel BS, Mathialagan S, Mnich SJ, Nemirovskiy OV, Carter J, Gierse JK, Wang JL, Vazquez ML, Moore WM, Masferrer JLDistinction of microsomal prostaglandin E synthase-1 (mPGES-1) inhibition from cyclooxygenase-2 inhibition in cells using a novel, selective mPGES-1 inhibitorBiochem Pharmacol.(2010 May 15)
57.^Xu D, Rowland SE, Clark P, Giroux A, Côté B, Guiral S, Salem M, Ducharme Y, Friesen RW, Méthot N, Mancini J, Audoly L, Riendeau DMF63 {2-(6-chloro-1H-phenanthro{9,10-d}imidazol-2-yl)-isophthalonitrile}, a selective microsomal prostaglandin E synthase-1 inhibitor, relieves pyresis and pain in preclinical models of inflammationJ Pharmacol Exp Ther.(2008 Sep)
58.^Bruno A, Di Francesco L, Coletta I, Mangano G, Alisi MA, Polenzani L, Milanese C, Anzellotti P, Ricciotti E, Dovizio M, Di Francesco A, Tacconelli S, Capone ML, Patrignani PEffects of AF3442 {N-(9-ethyl-9H-carbazol-3-yl)-2-(trifluoromethyl)benzamide}, a novel inhibitor of human microsomal prostaglandin E synthase-1, on prostanoid biosynthesis in human monocytes in vitroBiochem Pharmacol.(2010 Apr 1)
60.^Kalinski PRegulation of immune responses by prostaglandin E2J Immunol.(2012 Jan 1)
61.^Trappe TA, Standley RA, Jemiolo B, Carroll CC, Trappe SWProstaglandin and myokine involvement in the cyclooxygenase-inhibiting drug enhancement of skeletal muscle adaptations to resistance exercise in older adultsAm J Physiol Regul Integr Comp Physiol.(2013 Feb)
64.^Tober KL, Thomas-Ahner JM, Maruyama T, Oberyszyn TMPossible cross-regulation of the E prostanoid receptorsMol Carcinog.(2007 Aug)
65.^Sugimoto Y, Narumiya SProstaglandin E receptorsJ Biol Chem.(2007 Apr 20)
72.^Sliwinski S, Croonenberghs J, Christophe A, Deboutte D, Maes MPolyunsaturated fatty acids: do they have a role in the pathophysiology of autismNeuro Endocrinol Lett.(2006 Aug)
73.^Vancassel S, Durand G, Barthélémy C, Lejeune B, Martineau J, Guilloteau D, Andrès C, Chalon SPlasma fatty acid levels in autistic childrenProstaglandins Leukot Essent Fatty Acids.(2001 Jul)
74.^Bell JG, Miller D, MacDonald DJ, MacKinlay EE, Dick JR, Cheseldine S, Boyle RM, Graham C, O'Hare AEThe fatty acid compositions of erythrocyte and plasma polar lipids in children with autism, developmental delay or typically developing controls and the effect of fish oil intakeBr J Nutr.(2010 Apr)
78.^Okuda S, Saito H, Katsuki HArachidonic acid: toxic and trophic effects on cultured hippocampal neuronsNeuroscience.(1994 Dec)
79.^Dehaut F, Bertrand I, Miltaud T, Pouplard-Barthelaix A, Maingault Mn-6 polyunsaturated fatty acids increase the neurite length of PC12 cells and embryonic chick motoneuronsNeurosci Lett.(1993 Oct 29)
81.^Fukaya T, Gondaira T, Kashiyae Y, Kotani S, Ishikura Y, Fujikawa S, Kiso Y, Sakakibara MArachidonic acid preserves hippocampal neuron membrane fluidity in senescent ratsNeurobiol Aging.(2007 Aug)
82.^Okaichi Y, Ishikura Y, Akimoto K, Kawashima H, Toyoda-Ono Y, Kiso Y, Okaichi HArachidonic acid improves aged rats' spatial cognitionPhysiol Behav.(2005 Mar 31)
83.^Kotani S, Nakazawa H, Tokimasa T, Akimoto K, Kawashima H, Toyoda-Ono Y, Kiso Y, Okaichi H, Sakakibara MSynaptic plasticity preserved with arachidonic acid diet in aged ratsNeurosci Res.(2003 Aug)
84.^Ishikura Y, Ikeda G, Akimoto K, Hata M, Kusumoto A, Kidokoro A, Kontani M, Kawashima H, Kiso Y, Koga YArachidonic acid supplementation decreases P300 latency and increases P300 amplitude of event-related potentials in healthy elderly menNeuropsychobiology.(2009)
85.^Martini R, Fischer S, López-Vales R, David SInteractions between Schwann cells and macrophages in injury and inherited demyelinating diseaseGlia.(2008 Nov 1)
86.^Cámara-Lemarroy CR, Guzmán-de la Garza FJ, Barrera-Oranday EA, Cabello-García AJ, García-Tamez A, Fernández-Garza NECelecoxib accelerates functional recovery after sciatic nerve crush in the ratJ Brachial Plex Peripher Nerve Inj.(2008 Nov 26)
87.^Camara-Lemarroy CR, Gonzalez-Moreno EI, Guzman-de la Garza FJ, Fernandez-Garza NEArachidonic acid derivatives and their role in peripheral nerve degeneration and regenerationScientificWorldJournal.(2012)
88.^Nakano D, Ishii F, Fujii K, Ishikura Y, Akimoto K, Kontani M, Kawashima H, Kiso Y, Matsumura YEffects of dietary arachidonic acid supplementation on age-related changes in endothelium-dependent vascular responsesJ Nutr Sci Vitaminol (Tokyo).(2007 Feb)
89.^Oe H, Hozumi T, Murata E, Matsuura H, Negishi K, Matsumura Y, Iwata S, Ogawa K, Sugioka K, Takemoto Y, Shimada K, Yoshiyama M, Ishikura Y, Kiso Y, Yoshikawa JArachidonic acid and docosahexaenoic acid supplementation increases coronary flow velocity reserve in Japanese elderly individualsHeart.(2008 Mar)
90.^Zhou L, Nilsson ASources of eicosanoid precursor fatty acid pools in tissuesJ Lipid Res.(2001 Oct)
92.^Andersson A, Sjödin A, Hedman A, Olsson R, Vessby BFatty acid profile of skeletal muscle phospholipids in trained and untrained young menAm J Physiol Endocrinol Metab.(2000 Oct)
93.^Helge JW, Wu BJ, Willer M, Daugaard JR, Storlien LH, Kiens BTraining affects muscle phospholipid fatty acid composition in humansJ Appl Physiol.(2001 Feb)
95.^Mo C, Romero-Suarez S, Bonewald L, Johnson M, Brotto MProstaglandin E2: from clinical applications to its potential role in bone- muscle crosstalk and myogenic differentiationRecent Pat Biotechnol.(2012 Dec)
96.^Palmer RMProstaglandins and the control of muscle protein synthesis and degradationProstaglandins Leukot Essent Fatty Acids.(1990 Feb)
98.^Vandenburgh HH, Hatfaludy S, Sohar I, Shansky JStretch-induced prostaglandins and protein turnover in cultured skeletal muscleAm J Physiol.(1990 Aug)
100.^Demers LM, Harrison TS, Halbert DR, Santen RJEffect of prolonged exercise on plasma prostaglandin levelsProstaglandins Med.(1981 Apr)
101.^Karamouzis M, Langberg H, Skovgaard D, Bülow J, Kjaer M, Saltin BIn situ microdialysis of intramuscular prostaglandin and thromboxane in contracting skeletal muscle in humansActa Physiol Scand.(2001 Jan)
102.^Vandenburgh HH, Hatfaludy S, Karlisch P, Shansky JSkeletal muscle growth is stimulated by intermittent stretch-relaxation in tissue cultureAm J Physiol.(1989 Mar)
104.^Roberts MD, Iosia M, Kerksick CM, Taylor LW, Campbell B, Wilborn CD, Harvey T, Cooke M, Rasmussen C, Greenwood M, Wilson R, Jitomir J, Willoughby D, Kreider RBEffects of arachidonic acid supplementation on training adaptations in resistance-trained malesJ Int Soc Sports Nutr.(2007 Nov 28)
105.^Trappe TA, White F, Lambert CP, Cesar D, Hellerstein M, Evans WJEffect of ibuprofen and acetaminophen on postexercise muscle protein synthesisAm J Physiol Endocrinol Metab.(2002 Mar)
106.^Trappe TA, Fluckey JD, White F, Lambert CP, Evans WJSkeletal muscle PGF(2)(alpha) and PGE(2) in response to eccentric resistance exercise: influence of ibuprofen acetaminophenJ Clin Endocrinol Metab.(2001 Oct)
107.^Sawada K, Kawabata K, Yamashita T, Kawasaki K, Yamamoto N, Ashida HAmeliorative effects of polyunsaturated fatty acids against palmitic acid-induced insulin resistance in L6 skeletal muscle cellsLipids Health Dis.(2012 Mar 12)
108.^Hommelberg PP, Plat J, Langen RC, Schols AM, Mensink RPFatty acid-induced NF-kappaB activation and insulin resistance in skeletal muscle are chain length dependentAm J Physiol Endocrinol Metab.(2009 Jan)
110.^Lee JS, Pinnamaneni SK, Eo SJ, Cho IH, Pyo JH, Kim CK, Sinclair AJ, Febbraio MA, Watt MJSaturated, but not n-6 polyunsaturated, fatty acids induce insulin resistance: role of intramuscular accumulation of lipid metabolitesJ Appl Physiol.(2006 May)
112.^Pickersgill L, Litherland GJ, Greenberg AS, Walker M, Yeaman SJKey role for ceramides in mediating insulin resistance in human muscle cellsJ Biol Chem.(2007 Apr 27)
113.^Chavez JA, Knotts TA, Wang LP, Li G, Dobrowsky RT, Florant GL, Summers SAA role for ceramide, but not diacylglycerol, in the antagonism of insulin signal transduction by saturated fatty acidsJ Biol Chem.(2003 Mar 21)
114.^Clifford PS, Hellsten YVasodilatory mechanisms in contracting skeletal muscleJ Appl Physiol.(2004 Jul)
115.^Giordano RM, Newman JW, Pedersen TL, Ramos MI, Stebbins CLEffects of dynamic exercise on plasma arachidonic acid epoxides and diols in human volunteersInt J Sport Nutr Exerc Metab.(2011 Dec)
117.^Agas D, Marchetti L, Hurley MM, Sabbieti MGProstaglandin F2α: a bone remodeling mediatorJ Cell Physiol.(2013 Jan)
118.^Gervais FG, Cruz RP, Chateauneuf A, Gale S, Sawyer N, Nantel F, Metters KM, O'neill GPSelective modulation of chemokinesis, degranulation, and apoptosis in eosinophils through the PGD2 receptors CRTH2 and DPJ Allergy Clin Immunol.(2001 Dec)
119.^Hirai H, Tanaka K, Yoshie O, Ogawa K, Kenmotsu K, Takamori Y, Ichimasa M, Sugamura K, Nakamura M, Takano S, Nagata KProstaglandin D2 selectively induces chemotaxis in T helper type 2 cells, eosinophils, and basophils via seven-transmembrane receptor CRTH2J Exp Med.(2001 Jan 15)
120.^Zhu H, Ma H, Ni H, Ma XH, Mills N, Yang ZMExpression and regulation of lipocalin-type prostaglandin d synthase in rat testis and epididymisBiol Reprod.(2004 Apr)
121.^Treister NS, Richards SM, Suzuki T, Jensen RV, Sullivan DAInfluence of androgens on gene expression in the BALB/c mouse submandibular glandJ Dent Res.(2005 Dec)
122.^Samuelsson B, Goldyne M, Granström E, Hamberg M, Hammarström S, Malmsten CProstaglandins and thromboxanesAnnu Rev Biochem.(1978)
123.^Johnstone MA, Albert DMProstaglandin-induced hair growthSurv Ophthalmol.(2002 Aug)
124.^Kvedar JC, Baden HP, Levine LSelective inhibition by minoxidil of prostacyclin production by cells in cultureBiochem Pharmacol.(1988 Mar 1)
126.^van Goor SA, Dijck-Brouwer DA, Hadders-Algra M, Doornbos B, Erwich JJ, Schaafsma A, Muskiet FAHuman milk arachidonic acid and docosahexaenoic acid contents increase following supplementation during pregnancy and lactationProstaglandins Leukot Essent Fatty Acids.(2009 Jan)
127.^Smit EN, Koopmann M, Boersma ER, Muskiet FAEffect of supplementation of arachidonic acid (AA) or a combination of AA plus docosahexaenoic acid on breastmilk fatty acid compositionProstaglandins Leukot Essent Fatty Acids.(2000 Jun)
128.^Sauerwald TU, Demmelmair H, Koletzko BPolyunsaturated fatty acid supply with human milkLipids.(2001 Sep)
129.^Smit EN, Martini IA, Mulder H, Boersma ER, Muskiet FAEstimated biological variation of the mature human milk fatty acid compositionProstaglandins Leukot Essent Fatty Acids.(2002 May-Jun)
130.^Kuipers RS, Fokkema MR, Smit EN, van der Meulen J, Boersma ER, Muskiet FAHigh contents of both docosahexaenoic and arachidonic acids in milk of women consuming fish from lake Kitangiri (Tanzania): targets for infant formulae close to our ancient dietProstaglandins Leukot Essent Fatty Acids.(2005 Apr)