Research Breakdown on Mucuna pruriens
Mucuna Pruriens is a plant from India, the Caribbean or Arfica. It is a climbing shrub that has the ability to grow white, lavender, or purple flowers and causes itching upon physical contact with the outer hairs and skin. Like most herbal supplements, it possess an anti-oxidant content.
Mucuna is actually a legume, and thus has a caloric content. Beside the nutraceutical content below, mucuna (velvet) beans contain 20-35% protein by total caloric content. Mucuna also contains protein digestive enzyme inhibitors, which can be reduced with processing. Soaking in bicarbonate (following by autoclaving or cooking) has been shown to reduce a wide variety of anti-nutrients in Velvet beans, but also reduces levodopa content, the active ingredient.
Mucuna Pruriens contains:
Tetrahydroisoquinoline alkaloids in dosages of 8-24mg/500g dried milled seeds, which may have interactions with u-opioid receptors based on their structures.
Serotonin and its precursor, 5-HTP
NN-dimethyltryptamine and 5-MeO-dimethyltryptamine (bufotenin) in the seed.
Mucunain, a compound that causes itches when skin comes into contact with Mucuna species
Various saponins, anthraquinones, flavonoids, terpenoids, cardiac glycosides and tannins
A glycoprotein inhibitor of protein digestive enzymes
Behenic Acid, a dietary fatty acid with low bioavailability
D-chiro Inositol (2.1+/-0.2mg/g) and two storage forms of it (galactose glycosides; 11.4 and 21.2mg/g), also myo-inositol (8.2+/-0.6)
Mature seeds contain typically 3.1-6.1% Levodopa, although up to 12.5% has been recorded. The leaves tend to contain around 0.5%. When looking at studies that take random marketed supplements off the market and analyze them, values tend to fluctuate in the lower range of 3.8-4.3%.
Tryptamine compounds (hallucinogenic compounds) are at rather low dosages in the leaves and non-existent in the seeds; 0.006% Dimethyltryptamine, 0.0025% 5-MeO-Dimethyltryptamine, and 0.003% Dimethyltryptamine Oxide. 5-MeO-Dimethyltryptamine (Bufotenin) has been noted to be as high as 4.14ug/g in the root, but was not present in the pod. Statistically different results were seen with 30g Mucuna having a larger AUC and Cmax and 15g Mucuna having significantly less of a half-life. Measured AUC of 3-O-methyldopa was not significantly different between standard treatment (24,267+/-4559 ng/h/mL) and 30g Mucuna (22,698+/-2833 ng/h/mL) but was significantly less for 15g Mucuna (20,292+/-283.3 ng/h/mL). This insinuates less potential DNA damage from copper ion excess with Mucuna relative to Levodopa. The general co-ingestion of anti-oxidants alongside metal chelators may confer additional protection. At least one human study on Mucuna Pruriens compared its results to standard Levodopa treatment and noted less occurance of dyskinesia. It is not sure whether this is due to the above protective mechanisms, or due to Levodopa from Mucuna Pruriens being more bioactive. Levodopa in Mucuna Pruriens appears to be 2-3x more bioactive (potent) when compared to the same dose of isolated Levodopa, which is hypothesized to be due to a currently unknown Dopamine Decarboxylase inhibitor in Mucuna Pruriens. Standard Levodopa therapy for Parkinsons tends to pair it with Carbidopa to inhibit this enzyme and prolong the effects of Levodopa.
Levodopa and Mucuna Pruriens both seem effective. In isolation, Mucuna Pruriens may be better (due to the fact that it, as a herb, is never a molecule in isolation), but no studies exist comparing Mucuna to Levodopa and Carbidopa. The above studies suggest that Mucuna can hold its ground against standard Levodopa/Carbidopa therapy, but doesn't suggest it is better. Testosterone was also increased in the seminal experimental groups (those with low sperm motility or count), and to a more significant degree. Testosterone levels in the control (no sperm problems, still infertile) group went from 4.49 ± 0.53 to 5.72 ± 0.36ng/mL. The hypothesized mechanism of action was through levodopa content, in which the increase in serum dopamine antagonizes (works against) prolactin's suppressive effect on libido and testosterone. In rats with type II diabetes, increases in testosterone have been seen with an oral dose of 200mg/kg bodyweight Mucuna Pruriens or in blend with two other aphrodisiac herbs.
Only one human study, and it increased testosterone in infertile men. It is not known whether it can increase testosterone in already fertile men. At 7 different oral dosages ranging between 5-100mg/kg bodyweight, blood glucose was reduced by 18.6%-55.4%. Previous studies on the subject matter noted clinically relevant benefits only after 15 days at an oral dose of 200mg/kg bodyweight in rats, the differences between the two studies, as mentioned by the chronologically later study, may be due to chance variation in growing conditions. Chronic usage of Mucuna for blood sugar reduction appears to be more potent than acute usage, although both are effective. An oral dose of 5mg/kg bodyweight in rats is associated with a 55.3% reduction in blood sugar after 12 weeks, but acutely reduced blood sugar by 18.6%. The efficacy of 50mg and 100mg/kg bodyweight are not significantly different from an oral dose of 5mg/kg bodyweight Glibenclamide (diabetic drug) in rats. Potent effects on suppressing blood glucose may appear in as little as one month, although it seems to be somewhat maxed out at this time as evidence by one study investigating Mucuna supplementation at 1 and 2 months, and finding reductions of 38.01% and 40.41% at 1 and 2 months, respectively. The blood glucose lowering effect does not require diabetes as a pre-requisite, and is effective in normal rats as well. its influences on nerve damage in diabetic rats does not appear to be significant. Although Mucuna Pruriens may suppress a rise in urinary albumin levels associated with diabetes, it fails to prevent the hypertrophy of the kidneys associated with diabetes. It is not known what causes these effects, but a hypothesis is the D-chiro inositol content. With a content of about 2.1+/-0.2mg/g in Mucuna Pruriens, the dosages used in the above studies nears 7mg which is in the lower range of effectiveness in studies looking at pure D-chiro Inositol. A rather large dose of Mucuna, 15-30g daily (4.86% Levodopa; ended up being 500-1000mg Levodopa daily), was equally effective as standard Levodopa/Carbidopa (200mg/50mg) treatment in treating symptoms of Parkinson's Disease. A rat study investigating the same question found that low dose Mucuna paired with benserazide (peripheral dopa-decarboxylase inhibitor) was able to suppress symptoms associated with Parkinsons while low dose Levodopa + Benserazide was not; additionally, long-term usage of Mucuna Pruriens was more effective than long-term usage of Levodopa in isolation, when both were contributing the same dose of Levodopa. The differences seen were suggested to be due to a possible Dopa-decarboxylase inhibitor in Mucuna Pruriens. Other studies comparing Levodopa to Mucuna also note this difference, and suggest that one needs thrice as much Levodopa in isolation to match Levodopa from Mucuna. Beyond the double blind study mentioned, three open label studies in humans have been conducted. Over a period of 12 weeks with 45g Mucuna Pruriens (1500mg Levodopa equivalent) was found to improve symptoms of Parkinsons. The levodopa content has carryover to other dopamine-related conditions, such as suppression of tardive/orofacial dyskinesia symptoms. Doses as low as 48mg/kg Mucuna in rats (6% Levodopa) reduces tacrine-induced spontaneous jaw movements, an animal model for tardive dyskinesia. This dosage is approximately 8mg/kg bodyweight after conversion to human dosages based on Body Surface Area, or 730mg daily for a 200lb person.
Mucuna Pruriens definitely appears to have interactions with Parkinson's Disease, although its exact role (treatment, adjunct therapy, preventative medicine) is not fully examined. It looks to be a highly promising herbal. Mucuna Pruriens was also found to restore levels of serotonin and catecholamines in the substantia nigra (area of the brain associated with Parkinson's), whereas isolated levodopa was not able to do this. This same dose also increases various parameters of male semen indicative of increased fertility. In regards to testosterone, one study noted it could be increased over 3 months after ingestion of 5g Mucuna Pruriens seed powder daily; however, this study was conducted in infertile men and the increases seen did not exceed control. In rat studies looking at sexuality, mounting frequency is significantly increased at 150-250mg/kg bodyweight in rats; a dose of 2.4-4mg/kg bodyweight in humans assuming standard Body Surface Area conversions. Similar results have been seen in rats with type II diabetes induced by streptozotocin at a dose of 200mg/kg bodyweight and the same dosage/animal model of diabetes was found to alleviate damage on penile tissue associated with diabetes, suggesting preservation of sexuality in response to a disease state.
Appears to be effective in increasing sperm parameters and libido in studies on infertile men, but the evidence for its usage in otherwise healthy persons is less robust. It might be effective, but it really isn't known for sure. This protection from venom appears to be mediated through a protease inhibitor; normally seen as a negative due to preventing absorption of dietary protein, the specific glycoprotein in Mucuna Pruriens can degrade the toxin from this snake as said toxins are quaternary proteins. Mucuna Pruriens shows efficacy against EC toxin either when pre-loaded or as a single bolus in rats. It belongs to the Kunitz family of trypsin inhibitors. The glycoprotein inhibitor appears to be most stable at pHs of 4-7 and have structural similarities to the protease inhibitor found in soy. The inhibitory amounts are maxed at 61.5% for trypsin and 47.9% for chymotrypsin, with slightly higher values for whole Mucuna protein extract rather than isolated glycoprotein. All inhibitory potential is lost at 100°C, and efficacy starts to be lost at 50°C, Increasing the pH to 9-12 can destroy the protein at temperatures of 75°C. Protein digestability also increases when exposed to irradation in a dose-dependent manner. Irradiation may decrease Manganese and Sodium content slightly and reduced caloric content on a basis of dry weight slightly.
The above trypsin inhibitor is the same kind of 'evil' compound found in foods like soy that reduce protein absorption, and thus cooking Mucuna beans would be smart for the protein content. If you are buying powdered Mucuna Pruriens supplements, it would probably be prudent to look for roasted supplements, as cooking them normally destroys L-DOPA. The study on irradiation did not measure L-DOPA. In rats, doses of 32mg/kg or above are associated with 'some adverse side effects' (hyperventilation, reduced spontaneous motor activity, spontaneous erections) that appeared 1 hour after ingestion transiently, and doses up to 100mg/kg are free from more significant side effects for up to 12 weeks (study termination). This was thought to be due to women (of which constituted 85% of the affected) eating raw beans prior to sufficient preparation, and the results may have been a combination of protein deficiency paired with Levodopa, Bufotenin and N,N-dimethyltryptamine (all of which are constituents of Mucuna Pruriens). It is highly unlikely these results can be extrapolated to first world supplementation.
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