A bunch of 'plant steroid' molecules (like ecdysteroids are insect steroids) that are present in relatively high amounts in mustard; still nowhere near enough to get jacked off of mustard. Have not been shown to be effective in humans yet, remains an unexplored research field.

Our evidence-based analysis features 9 unique references to scientific papers.

Research analysis by and verified by the Research Team. Last updated on Oct 2, 2018.

Summary of Brassinosteroids

Primary Information, Benefits, Effects, and Important Facts

Brassinosteroids are a class of steroid compounds found in plants, used to regulate and induce plant growth. They share many similarities with human steroid hormones, but are generally at too low of a dose to exert effects in humans (on hormonal profiles) via food consumption.

They have been implicated in mouse models on skeletal muscle growth, and in some cell lines show promise at being anti-carcinogens (cancer protective).

How to Take

Recommended dosage, active amounts, other details

There is not enough information for a recommend dosage at this time.

1Sources and structure

Brassinosteroids are polyhydroxylated compounds related to the structure of 5a-cholestane, a chemical structure similar to many androgenic compounds. The chemical class of brassinosteroids share similar actions of mammalians steroids, but act in plants[1] mostly via genetically mediated factors.[2]

One of the main sources of brassinosteroids is in pollens in order to induce growth. They act in micromolar concentrations, which helps to explain a past yield of 10mg from 230kg pollen.[3][4] Brassinosteroids can be synthesized in laboratory settings.[5][6]

2Muscle protein synthesis

In does of 20-60mg/kg bodyweight, a brassinosteroid found in mustard (28-Homobrassinolide, or 28-HB) was able to stimulate protein synthesis and concomitantly inhibit breakdown of muscle while increasing strength. The mode of adminstration was oral consumption via Akt phosphorylation, and the results were somewhat confounded with increases in food intake, although the magnitude of which is not causative of the differences seen in lean mass.[7] No binding to the Androgen Receptor (AR) was noted, and no changes in serum testosterone noted.


Brassinosteroids have been implicated in inhibiting cancer growth at very low (possibly dietary) levels.[8][9]

Scientific Support & Reference Citations


  1. Asami T, Nakano T, Fujioka S. Plant brassinosteroid hormones. Vitam Horm. (2005)
  2. Müssig C, Fischer S, Altmann T. Brassinosteroid-regulated gene expression. Plant Physiol. (2002)
  3. Mitchell JW, et al. Brassins--a new family of plant hormones from rape pollen. Nature. (1970)
  4. Brassinolide, a plant growth-promoting steroid isolated from Brassica napus pollen.
  5. New Route for the Synthesis of (22S,23S)-28-Homobrassinolide.
  6. Acebedo SL, et al. Synthesis and biological activity of ring-A difluorinated brassinosteroids. Steroids. (2011)
  7. Esposito D, et al. Anabolic effect of plant brassinosteroid. FASEB J. (2011)
  8. Malíková J, et al. Anticancer and antiproliferative activity of natural brassinosteroids. Phytochemistry. (2008)
  9. Steigerová J, et al. Brassinosteroids cause cell cycle arrest and apoptosis of human breast cancer cells. Chem Biol Interact. (2010)

Cite this page

"Brassinosteroids,", published on 11 December 2012, last updated on 2 October 2018,