Studies related to HDL-C and Red Clover Extract

Influence Of Red Clover-derived Isoflavones On Serum Lipid Profile In Postmenopausal Women

Effect Increase
Trial Design Double blind
Trial Length 6+ Months
Number of Subjects 96
Gender Female
Age Range 45-64
Body Types Overweight
Notes for this study:
Supplementation of 40mg isoflavones from red clover daily over a period of one year was able to increase HDL cholesterol (19%) and reduced LDL (17.5%) and total cholesterol (12.5%) while the influence on triglycerides (11.5%) failed to reach significance; while the aforementioned numbers refer to the 12 month point, benefits were seen after three months and persisted until 12 months at a similar magnitude.

Isoflavones From Red Clover Improve Systemic Arterial Compliance But Not Plasma Lipids In Menopausal Women

Effect None
Trial Design Cohort
Trial Length 1-6 months
Number of Subjects 17
Gender Female
Age Range 45-64
Body Types Overweight, Average
Notes for this study:
As assessed by ultrasound, red clover extract (40-80mg isoflavones) appears to increase arterial compliance by up to 23% in otherwise healthy postmenopausal women despite no influence on circulating lipids relative to placebo group (randomization had 5-fold more women in active treatment than placebo, and the sample size was only 17).

(Rating of C given to this study due to the low sample in placebo group and a high dropout rate of 35%)
Funding issues for this study:
Supplies provided by Novagen, study not funded by Novagen

A Biochanin-enriched Isoflavone From Red Clover Lowers LDL Cholesterol In Men

Effect None
Trial Design Double blind
Trial Length 1-6 months
Number of Subjects 80
Gender Both Genders
Age Range 45-64
Body Types Overweight, Average
Notes for this study:
Supplementation of Red Clover isoflavones (80mg of biochanin A and formononetin in a 3.5:1 ratio) over six weeks in older subjects was able to reduce LDL-C by 9.5% (0.38mM) in men, but appeared to have no effect in women; this may be somewhat influenced to the randomization (no significant differences, but treatmented trended to have higher baseline LDL-C) and no other lipid parameters were measured.

Effect Of Trifolium Pratense-derived Isoflavones On The Lipid Profile Of Postmenopausal Women With Increased Body Mass Index

Effect None
Trial Design Double blind
Trial Length 1-6 months
Number of Subjects 53
Gender Female
Age Range 45-64
Body Types Obese, Overweight, Average
Notes for this study:
80mg of red clover isoflavones (MF11RCE) daily for ninety days in postmenopausal women failed to influence any parameter of lipid metabolism in women with a BMI less than 25, while in those above this weight point there was a significant reduction in apolipoprotein A (63%, but placebo was reduced 42%), total cholesterol (4.7%), and LDL cholesterol (15.7%) with no change in triglycerides nor HDL-C.
Funding issues for this study:
Funded by the producers of MF11RCE, Melbrosin Ind.

Effect Of Isoflavones On Lipids And Bone Turnover Markers In Menopausal Women

Effect None
Trial Design Double blind
Trial Length 1-6 months
Number of Subjects 252
Gender Female
Age Range 45-64
Body Types Overweight
Notes for this study:
Supplementation of 40mg Promensil twice daily failed to significantly influence all biomarkers of circulating cholesterol, although there appeared to be a slight reduction in triglycerides in the whole group (9.7%) which was significantly higher with those having higher baseline triglycerides. Biomarkers of bone metabolism (urinary N-Tx and serum osteocalcin) were not changed significantly.

The Effect Of Promensil, An Isoflavone Extract, On Menopausal Symptoms

Effect Increase
Trial Design Double blind
Trial Length 1-6 months
Number of Subjects 37
Gender Female
Age Range 45-64
Body Types Overweight
Notes for this study:
Supplementation of 40mg Promensil, but not 160mg, was able to increase HDL-C in postmenopausal women by 18% after twelve weeks, but no other biochemical marker nor menopausal symptom appeared to be influenced.
Funding issues for this study:
Funded by Novagen, producers of Promensil

The Effects Of Dietary Supplementation With Isoflavones From Red Clover On The Lipoprotein Profiles Of Post Menopausal Women With Mild To Moderate Hypercholesterolaemia

Effect None
Trial Design Double blind
Trial Length 1-6 months
Number of Subjects 75
Gender Female
Age Range 45-64
Body Types Overweight
Notes for this study:
In a preliminary study where postmenopausal women with high cholesterol were given active treatment (40-80mg of red clover isoflavones) or placebo in a 6:1 ratio, supplementation over 12 weeks failed to significantly reduce circulating cholesterol or triglycerides and also failed to alter blood pressure or weight.
Funding issues for this study:
Funded by Novagen, producers of Promensil

Modest Protective Effects Of Isoflavones From A Red Clover-derived Dietary Supplement On Cardiovascular Disease Risk Factors In Perimenopausal Women, And Evidence Of An Interaction With ApoE Genotype In 49-65 Year-old Women

Effect None
Trial Design Double blind
Trial Length 6+ Months
Number of Subjects 177
Gender Female
Age Range 45-64
Body Types Overweight
Notes for this study:
43.5mg of red clover isoflavones daily for one year has failed to significantly improve cardiovascular risk factors, and when controlling for the ApoE genotype there was a stronger trend for inprovement that still failed to reach statistical significance.

Effects Of Supplementation With Purified Red Clover (Trifolium Pratense) Isoflavones On Plasma Lipids And Insulin Resistance In Healthy Premenopausal Women

Effect None
Trial Design Double blind
Trial Length 1-6 months
Number of Subjects 31
Gender Female
Age Range 18-29, 30-44
Body Types Average
Notes for this study:
Oral supplementation of 80mg Promensil for three menstrual cycles in otherwise healthy women failed to significantly alter any biomarker of glucose or lipid metabolism measured despite urinary isoflavones increasing 15-fold.

Effects Of Dietary Supplementation With Isoflavones From Red Clover On Ambulatory Blood Pressure And Endothelial Function In Postmenopausal Type 2 Diabetes

Effect None
Trial Design Double blind
Trial Length 2-4 Weeks
Number of Subjects 16
Gender Female
Age Range 45-64, 65+
Body Types Obese, Overweight
Notes for this study:
Oral supplementation of 50mg isoflavones (mostly formononetin) from red clover for four weeks in postmenopausal women with type II diabetics was able to improve basal blood flow as assessed by forearm resistance (L-NMMA treatment) and reduced blood pressure by 8mmHg systolic and 4.3mmHg diastolic. No statistically significant changes were noted elsewhere.

Effect Of Red Clover-derived Isoflavone Supplementation On Insulin-like Growth Factor, Lipid And Antioxidant Status In Healthy Female Volunteers: A Pilot Study

Effect Increase
Trial Design Double blind
Trial Length 2-4 Weeks
Number of Subjects 23
Gender Female
Age Range 18-29, 30-44, 45-64
Body Types Overweight, Average
Notes for this study:
Supplementation of 40mg Promensil twice daily over the course of one month was able to slightly increase HDL-C concentrations () while there was no influence on IGF or any of its binding proteins, although when excluding postmenopausal women from the analysis there was a trend to reduce IGF-1 only.

The Effect Of Isoflavones Extracted From Red Clover (Rimostil) On Lipid And Bone Metabolism

Effect Increase
Trial Design Cohort
Trial Length 1-6 months
Number of Subjects 50
Gender Female
Age Range 45-64
Body Types Overweight
Notes for this study:
Supplementation of Rimostil (from Red Clover) has been noted to improve HDL cholesterol levels by 15.7-28.6% with the dosage range of 28.5-85.5mg, with most efficacy coming from the middle dose of 57mg. There was a significant decrease in apolipoprotein B (11.5-17%) and increase in bone mineral density of 3.1-4%, although the latter was not seen at 28.5mg daily.

While this study was double-blinded, there was not a placebo control used alongside the double-blinded experimental group.

The Effect Of Red Clover Isoflavones On Menopausal Symptoms, Lipids And Vaginal Cytology In Menopausal Women: A Randomized, Double-blind, Placebo-controlled Study

Effect None
Trial Design Double blind
Trial Length 1-6 months
Number of Subjects 53
Gender Female
Age Range 45-64, 65+
Body Types Overweight, Average
Notes for this study:
Supplementation of 80mg of red clover isoflavones daily to postmenopausal women for 90 days was able to reduce symptoms of menopause with minimal effects on the cardiovascular system nor the hormonal system.