Tribulus terrestris

Tribulus terrestris is a plant from Ayurveda where the root and fruits are used for male virility and general vitality, respectively. The roots enhance libido and sexual well being without affecting testosterone while the fruits appear to be potently protective of organ function.

This page features 95 unique references to scientific papers.


Confused about what actually Works?
MUST GET: Supplement Stack Guides - Saving You Money & Time

   

Tribulus terrestris is a herb from Ayurveda that is mostly recommended for male health including virility and vitality, and specifically more catered towards cardiovascular and urogenital health. It is a common supplement for its libido enhancing properties and supposed testosterone boosting properties.

On the sexual side of things, tribulus does appear to be a relatively reliable and potent libido enhancer in rats and the lone human study assessing this has confirmed an increase in sexual well being and erectile function. While it is not exactly known how tribulus works, it is known to enhance androgen receptor density in the brain (muscle tissue not confirmed) which may enhance the libido enhancing properties of androgens. Limited evidence suggests that it is weak to non-effective in enhancing fertility.

A specific component, tribulosin, appears to be quite potently cardioprotective and is effective in the 1-10nM range. It has not yet been tested in living creatures, but remains a very promising option.

In animal research, the fruits of tribulus appears to protect the organs (mostly liver and kidneys) from oxidative damages at reasonably low dosages and also exert anti-stress effects; confirming the status of tribulus terrestris as an Adaptogen.

Despite the above promise as an adaptogen and a libido enhancer, studies investigating tribulus in sports performance have all failed to find benefits. The herb seems to be a possibly healthy herb that enhances sexuality but with limited use for power output and testosterone enhancement (which it has repeatedly failed to do).

Follow this Page for updates

Confused about Supplements?
Get the Stack Guides

Also Known As

Trib, puncturevine, protodioscin


Things to Note

  • Tribulus Terrestris has a highly 'earthy' adverse taste to the powder

Is a Form of


Stacks Part Of increased usefulness


Caution Notice

Examine.com Medical Disclaimer

Given a 60% saponin extract, a dose of between 200-450mg a day is typically used for libido enhancement and sexuality. If rodent research applies to humans, then the dosage of 5mg/kg of tribulus terrestris saponins which is seen as the optimal dose would correlate to a human dose of:

  • 55 mg saponins (90 mg of a 60% extract)
  • 70 mg saponins (120 mg of a 60% extract)
  • 90 mg saponins (150 mg of a 60% extract)

Which suggests low doses are better. If a concentrated extract is not used, traditional dosages of the basic root powder are in the 5-6g range while the fruits are in the 2-3g range.


The Human Effect Matrix looks at human studies (excluding animal/petri-dish studies) to tell you what effect Tribulus terrestris has in your body, and how strong these effects are.
GradeLevel of Evidence
ARobust research conducted with repeated double blind clinical trials
BMultiple studies where at least two are double-blind and placebo controlled
CSingle double blind study or multiple cohort studies
DUncontrolled or observational studies only
Level of Evidence
EffectChange
Magnitude of Effect Size
Scientific ConsensusComments
BTestosterone

In otherwise healthy males, testosterone is not influenced with supplementation of tribulus terrestris. There may be an increase in infertile men, but this is weak.

CLean Mass

Insufficient evidence to support an increase of lean mass associated with tribulus relative to placebo during a training program.

CFat Mass

No significant influences on fat mass are noted with tribulus terrestris

CPower Output

No significant alterations in power output associated with tribulus supplementation.

CLuteinizing Hormone

No influence on luteinizing hormone has been detected with supplemental tribulus

CFatigue

Exercise related fatigue and vigor is unaffected by tribulus supplementation in trained men

CBlood Pressure

Minor

A decrease in blood pressure has been noted in hypertensive subjects; insufficient evidence to address efficacy in normotensive persons

CHeart Rate

Minor

A decrease in heart rate has been observed in hypertensive persons given tribulus supplementation

CTotal Cholesterol

Minor

A decrease in cholesterol levels has been noted with tribulus supplementation

CDiuresis

Minor

3g of the fruits or a water extract thereof appears to increase overall urine volume after a month of supplementation by around 200mL daily.

CErections

Minor

In infertile men, supplementation of 6g tribulus root appears to increase rigidity of erections and improve performance (reduce anxiety while delaying ejaculation and improving... show

CLibido

Minor

There appears to be an increase in frequency of coitus and improvement in sexual well being associated with supplementation of 6g of the basic root extract of tribulus.

CSperm Count

The increase in sperm count seen with tribulus failed to outperform placebo.

CSperm Quality

The increase in sperm quality seen with 6g tribulus root has failed to outperform placebo in infertile men.


Studies Excluded from Consideration

  • Confounded with Murraya koenigii[1]
  • Confounded with N-Glucosamine, D-Acetyl-N-Glucosamine, and Ecklonia bicyclis[2][3]

Disagree? Join the Tribulus terrestris Discussion

Table of Contents:


Edit1. Source and Composition

1.1. Sources

Tribulus terrestris (of the family Zygophyllaceae) is an annual creeping herb widespread in China, eastern Asia, and extends into western Asia and southern Europe. The fruits of this plant have been used in Traditional Chinese Medicine for the treatment of eye trouble, edema, abdominal distention, high blood pressure, and cardiovascular diseases while in India its usage in Ayurveda was for the purpose of impotency, poor appetite, jaundice, urogenital disorders, and cardiovascular diseases.[4][5][6][7][4] In Ayurvedic medicine, Tribulus terrestris is referred to as Gokshura or Chhota Gokshura for the treatment of lower back pain, sciatica, inflammation of the pelvic and sacral region, dry cough, respiratory disorders;[8][9] it is known to be interchangeable with Pedalium murex for the purpose of gokshura.[9]

Tribulus is a plant used in both traditional chinese medicine and ayurveda for purposes mostly related to cardiovascular health, virility, and urogenital health

The tribulus genera extends to 25 species of which 12 of them are found in the middle east.[10]

There are other species in the tribulus genera that are sometimes used in supplementation, including pentandrus (has more Kaempferol in its flavonoid profile whereas terrestris has more Quercetin; pentandrus also has tricin-7-diglucoside as a unique componnet)[11] and alatus which also possesses steroidal glycosides such as spirostane, furostane, and cholestane[12] and is implicated in testosterone boosting[13] similar to Pedalium murex but not tribulus terrestris.

Tribulus terrestris is one of a few species of the tribulus genera and the most commonly supplemented species

1.2. Composition

The main components of the tribulus terrestris root extract include:

  • Protodioscin[14] commonly seen as the main bioactive and can comprise up to 45% of the Tribulus terrestris dry extract (exluding macronutrient and water weight)
  • Pseudoprotodioscin[14]
  • Dioscin[14] and Diosgenin (0.031% in roots (wet weight); 0.065% in stem; 0.16% in aerial parts, and 0.095% overall)[15]
  • Tribulosin (tigogenin 3-O-β-d-xylopyranosyl(1–2)-{β-d-xylopyranosyl (1–3)}-β-d-glucopyranosyl(1–4)-{a-l-rhamnopyranosyl(1–2)}-β-d-galactopyranoside), a spiral vagina steroid[14][16]
  • Hecogenin-3-O-β-d-glucopyranosyl(1 → 4)-β-d-galactopyranoside[17]
  • Gitogenin type saponins called Protodibestin and tribestin[14]
  • Sulphated spirostanol and furostanol saponins (Bulgarian only)[18] and general spirstanol and furostanol saponins[19]
  • di-p-coumaroylquinic acid and 4,5-di-p-cis-coumaroylquinic acid[20]
  • A 60kDa antilithiatic protein[21]
  • Vitamin C at 909mg/100g (0.9%)[22]

The fruits of the Tribulus plant (Tribulus terrestris fructi) contain:

  • Furostanol based steroidal saponins such as terrestroside A,B as well as terrestrinin B, terrestroneoside A, and chloromaloside[23][24][25] and glycosides of these molecules including tribufuroside C[5] and I-J[26]
  • Spirostane based steroidal saponins[27][28]
  • Tribulusamides A-D[29][30][31]
  • Terrestramide[30]
  • Pregnane glycosides[28]

The root contains phenolics at around 178.57mg/g in the water extract (17.8%) and 114.28mg/g in ethanolic (11.4%) with the flavonoid component being 10mg/g and 5mg/g, respectively.[22]

Both the fruits and the plant root itself appear to contain a variety of compounds that are roughly based on a steroid skeleton, steroidal saponins

1.3. Variability

Protodioscin, seen as the active muscle building compound (Tribulus' most heavily marketed claim), is highest in Turkey (Ankara), Bulgarian (Haskovo hirsut) and Macedonian (Bogdanzi glabr) respectively. These three species have more than double all other species.[14]

The chemical make-up of the plant seems to vary by region of growth, with Vietnamese and Indian tribulus lacking both tribestin and prototribestin (the gitogeninic type)and having higher levels of tribulosin, at around 50-150 times more than the aforementioned species.[14][32]

Much of the literature focuses on the extracts of Bulgarian, Chinese, and Indian origin;[14] which proves problematic if the tribestin or protodioscin contents are the active components.

Although the region of Tribulus growth appears to influence the components, the major difference is in those grown in middle-eastern Europe and Western Asia having a higher protodioscin content and those grown in Eastern Asia and Oceania tending to have greater tribuloin content and no gitogenic saponins

Beyond the numerical differences of the above 6 compounds, Bulgarian Tribulus is the only extract to possess sulphated spirostanol and furostanol saponins[18] and chinese tribulus seems to be the only compound with cisA/B ring junctures in their saponins.[14]

1.4. Formulations

Tribulus terrestris is sometimes paired with Boerhaavia diffusa as a formulation called Unex.[33]

When paired with Alga Ecklonia Bicyclis (80mg), D-glucosamine and N-acetyl-D-Glucosamine (100mg), and tribulus at 100mg the combination is known as Tradamixina.[2][3]


Edit2. Pharmacology

2.1. Distribution

Oral ingestion of 50mg/kg hydroalcoholic extract of tribulus terrestris (45.84% protodioscin) for eight weeks in rats has been noted to increase levels in the kidney tissue by 49ng/g (healthy rats) and 9ng/g (diabetic rats) which correlated with serum levels of 170ng/mL and 6ng/mL.[34]


Edit3. Neurology

3.1. Cholinergic Neurotransmission

Tribulus terrestris failed to inhibit acetylcholinesterase at 10µg/mL and very weakly inhibited it at 1mg/mL (37.89 ± 0.77) whereas this concentration range was able to inhibit butyrylcholinesterase by 17.06+/-0.55% (10µg/mL) and 78.32+/-1.27% (1mg/mL).[35]

Technically may interact with acetylcholine metabolism, but this has not yet been shown in a living model and it doesn't seem to be overly potent at the concentrations expected to be seen in the blood and brain

3.2. Appetite

One rat study investigating aphrodisia and using doses of 2.5mg/kg, 5mg/kg, and 10mg/kg tribulus terrestris extract noted increases in bodyweight in all groups with 5mg/kg bodyweight responding best; this study did not record food intake specfically.[36]

In a rat model of chronic manageable stress (induces depression), 750-2,250mg/kg of a tribulus terrestris extract (80% saponins) was able to preserve the reduction in appetite.[37]

There do appear to be increases in appetite in stressed rats and may be some inhernetly; needs more research to properly assess the traditional claims of increasing appetite

3.3. Analgesia

At least one study has indicated that Tribulus may possess pain-reducing properties (in accordance with traditional usage in Iran and surrounding regions).[38] In this study subjected mice to a chemical and thermal pain test (formalin and tail-flick, respectively) and although it was tested at various doses (50, 100, 200, 400, and 800mg/kg) an injection of 100mg/kg Tribulus extract appeared to be most potent and tended to be less pain-relieving than 2.5mg/kg morphine yet more than 300mg/kg Aspirin; the mechanism of action is not mediated by opioid receptors.[38]

In diabetic rats, 100-300mg/kg of a water extract over eight weeks is able to prevent diabetes-induced hyperalgesia (more sensitivity to pain) in heat and chemical testing with a potency comparable to 100mg/kg Pregabalin.[39]

Tribulus terrestris extracts appear to be fairly effective in reducing pain perception in response to chemical and heat pain tests, with a potency comparable to some reference drugs

3.4. Depression

In chronic stress induced depression given 375-2,250mg/kg tribulus terrestris (80% saponins) noted dose-dependent antistress effects with the highest dose fully normalizing the increases in corticosterone, adrenocorticotropic hormone, and corticotropin releasing factor.[37] 750mg/kg was as effective as the clinical dose of fluoxetine (1.8mg/kg).[37]

Ghana tablets (tribulus, Tinospora cordifolia, and Emblica officinalis) at 260mg/kg, correlating to a 2g human dose, appears to exert antidepressant and anxiolytic effects although to a degree weaker than the reference drugs diazepam (2mg/kg) and imipramine (5mg/kg).[8]

Tribulus terrestris appears to have antidepressant effects in stressed rats, suggesting that it is an adaptogenic herb. The potency is respectable although it requires very high doses to be so

3.5. Menopausal Symptoms

A study has been reported (vicariously through this review, not found online[40]) that usage of tribulus terrestris was associated with improvements in menopausal symptoms in 98% of the research group (49/50) although it had a potential conflict of interest.

The aphrodisiac/adaptogenic effects are supposed to extend to women, but this is not a well researched topic at all


Edit4. Cardiovascular Health

4.1. Cardiac Tissue

Tribulus terrestis saponins (100mg/L) are able to reduce infarct size in hearts exposed to ischemia/reperfusion and oxidation in vitro,[41][42] which is due to the tribulosin content (decreased infarct size to 20.24-30.32% of control with 1-100nM) in a manner that is abolished by blocking PKCε[42] or ERK signalling at 10nM;[16] as both the PKCε pathway[43] and ERK signalling[44] are involved in cardiomyocyte apoptosis, it is thought that tribulosin exerts a direct anti-apoptotic effect (specifically, by activating ERK which then translocates PKCε[16]).

Other saponins may have cardioprotective effects, such as hecogenin-3-O-β-d-glucopyranosyl(1 → 4)-β-d-galactopyranoside (30-100μg/mL) via activating PKCε[17] although they are significantly weaker than tribulosin at doing so.

Due to activating protein kinase C epsilon (PKCε), tribulosin can protect cardiac cells from cell death. This occurs at a remarkably low concentration and is likely relevant to oral supplementation of tribulus terrestris saponins

In hypertensive humans given 3g of tribulus fruits or a water extract thereof, four weeks supplementation was sufficient to slightly reduced heart rate.[45]

4.2. Blood Pressure

Tribulus terrestris appear to have ACE inhibitory properties in vitro at 0.33mg/mL 50-56% with the water extract (17-37% ethanolic and 11-28% acetone) with more potency in the aerial parts than the fruits.[46] In rats (spontaneously hypertensive 2K1C), 10mg/kg of the lyophilized fruit extract appears to reduce blood pressure and correct the higher than normal levels of ACE.[47]

In otherwise healthy rats, 2.5-10mg/kg tribulus terrestris appears to increase blood pressure in a dose-dependent manner (6-11%) during erections, although when the rats were not sexually stimulated there were no differences.[36]

Tribulus may reduce blood pressure via either the kidneys (a diuretic and/or protective effect) or via ACE inhibition, and while it shouldn't influence blood pressure in otherwise normal subjects it may increase the erection-induced blood pressure spike

In humans with hypertension, tribulus terrestris given at 2g of the whole fruit extract of a water extract thereof over four weeks noted significant reductions in systolic blood pressure (4-4.3%) by one week which increased to 9.6-10.7% after four weeks; diastolic blood pressure was reduced by 7.4-8.1% after four weeks.[45] This study should be approached with caution as the intervention group had significantly higher blood pressure at baseline which may have exacerbated the results.[45]

As the reduction in blood pressure was associated with an increase in urinary output, it is though that the observed benefits were secondary to the kidneys.[45]

Appears to reduce blood pressure in hypertensive humans, and while the lone study is incredibly promising more research should be put forward to ascertain the potency

4.3. Platelets

Tribulus pterocarpus phenolics have been noted to inhibit platelet aggregation induced by thrombin in a concentration dependent manner between 0.5-50µg/mL (40-70%) and inhibited overall blood platelet aggregation between 3-30%.[10] As the main bioactive for this effect was not delineated, it is unsure if this applies to tribulus terrestris.

Tribulus terrestris has been found to inhibit adrenaline-induced platelet aggregation with an IC50 value of 970µg/mL.[48]

Tribulus terrestris has weak and probably not relevant anti-platelet effects, although the species of pterocarpus appears to be quite potent. They were measured on different aggregators, however, and it is not sure if tribulus terrestris has a role in blood clotting or not

4.4. Cholesterol

A reduction in total cholesterol has been noted with tribulus terrestris fruits or a water extract thereof at 3g daily over four weeks, which reached 9.49-10.23% at the end of the study (placebo down 2.14%).[45]

Possible reductions in total cholesterol that need to be investigated further


Edit5. Interactions with Glucose Metabolism

5.1. Absorption

The seeds of tribulus terrestris appear to inhibit pancreatic α-amylase with an IC50 of 511µg/mL which was less effective than Turmeric isopropanol extraction (160ng/mL) and acetone extract (7.4µg/mL).[49] Concentrations of 0.1-10mg/mL of the isolated saponins have been noted to inhibit this enzyme activity by 20.83-52.62% in vitro[50] and there appears to be some inhibitory potential against α-glucosidase as well.[50]

In rats, 100mg/kg of the saponins from tribulus terrestris is able to approximately half the increase in blood glucose from an oral sucrose tolerance test; it failed to influence glucose absorption.[50]

May inhibit the enzymatic activity of pancreatic amylase and some other enzymes, and while it doesn't seem to be the most potent herb in doing so this may be biologically relevant

5.2. Diabetes

One study using 2g/kg Tribulus Terrestris in a model of streptozotocin-induced diabetes noted that it was as effective as 10mg/kg glibenclamide in normalizing one serum liver enzyme (AST) and more effective in normalizing ALT and serum creatinine.[51] This may have been secondary to prevention of damage to the liver, as assessed by histological examination.[51]

In diabetic rats, 50mg/kg of a hydroalcoholic extract (45.84% saponins) for eight weeks is able to improve insulin levels in serum with a potency comparable to 6mg/kg Olmesartan and slightly less effective than 1mg/kg Glimepiride.[34]

Appears to confer anti-diabetic effects, particularly insulin is increased (normally suppressed in these models of diabetes). Mechanisms are not yet known

5.3. Diabetic Adjuvants

Tribulus terrestris extract appears to have inhibitory potential against rat aldose reductase (IC50 of 348-444µg/mL) and polyphenol redutase (IC50 of 439-705µg/mL) with the water extract tending to be more potent than the ethanolic and chloroform extracts.[22] This inhibitory potential was lesser than other tested plants of Tinospora cordifolia (IC50 176µg/mL), Holy Basil (IC50 280µg/mL), and morinda cetrifolia (IC50 132µg/mL) as well as the standard reference Quercetin (IC50 82µg/mL) on aldose reductase.[22]

Tribulus terrestris appears to have aldose reductase inhibiting potential, which doesn't seem to be too potent when compared to other herbs

In diabetic rats, supplementation of 50mg/kg of an hydroalcoholic extract is able to improve kidney function as assessed by glomerular filtration rate.[34]

May improve kidney function in diabetic rats (diabetic nephropathy)


Edit6. Skeletal Muscle and Physical Performance

6.1. Glucose Metabolism

At least in diabetic rats, oral intake of 50mg/kg of a hydroalcoholic extract of tribulus terrestris for eight weeks failed to modify GLUT2 and GLUT4 concentrations in the soleus muscle.[34]

Does not appear to modify glucose uptake into skeletal muscle, although this is not a well researched topic

6.2. Power Output

In humans supplementing tribulus terrestris, resistance trained males using supplementation at 3.21mg/kg body weight (45% saponins) over eight weeks failed to experience an increase in power output[52] and supplementation of a standardized extract at 450mg (60% saponins) similarly failed in elite rugby players over five weeks.[53]

The limited human evidence has been conducted in well trained human subjects, and supplementation at the recommended doses for power output (and above the recommended dosages for libido enhancement) has twice failed to improve power output


Edit7. Inflammation and Immunology

7.1. Macrophages

Tribulus terrestris appears to be able to inhibit COX-2 induction in macrophages stimulated with LPS with 86.9% suppression at 10µg/mL although there was less suppression of iNOS (16.9%).[54]

Possibly relevant anti-inflammatory effects that need to be investigated further


Edit8. Interactions with Hormones

8.1. Testosterone

A tribulus terrestris extract (Bulgarian, thus higher protodioscin) seems to be able to increase androgen receptor (AR) immunoreactivity in the rat hypothalamus (specifically, the PVN) when dosed at 5mg/kg bodyweight for 8 weeks, increasing from 162.67+/-14.73 ARs to 383.17+/-15.73 ARs (58%) suggesting it may alter androgen signalling.[55] There was also a significant increase in neurons expressing NADPH-d by 67%, suggesting that Tribulus can increase Nitric Oxide Synthase (NOS) and subsequent NO production[55] as NADPH-d immunostaining can be a biomarker for NOS activity.[56]

Expression of the androgen receptor may be increased in the brain, possibly related to the libido enhancing effects

When testing for androgenic effects of Tribulus independent of serum testosterone levels, a rat study measuring prostate weight of rats subject to 11, 42, and 110mg/kg Tribulus extract (11.42% Protodioscin) with the lowest dose correlating to 750mg Tribulus extract in a 70kg human noted that, over 28 days of feeding, that aside from serum testoterone being unaffected prostatic weight was unaffected at all doses suggesting no intrinsic androgenic activity.[57]

There does not appear to be a direct androgenic effect of tribulus supplementation (independent of increasing testosterone)

When tested in vitro, an extract of tribulus terrestris did not appear to significantly inhibit the 5α-reductase enzyme.[58]

May not interact with the 5α-reductase enzyme

When looking at interventions, currently those that show an increase in circulating testosterone including a non-human primate injection of isolated protodioscin (7.5mg/kg) resulting in a 52% increase acutely which returned to baseline 90-180min after injection.[59] This same study noted that 8 weeks administration of 5-10mg/kg in rabbits and rats led to increases in dihydrotestosterone (DHT) in rabbits (testosterone increases statistically insignificant) and in the castrated rats testosterone increased by 25%[59] and another study exclusively using sexually sluggish rats (those that were previously shown to copulate less with receptive females) noted an increase in testosterone from 2.4+/-0.03ng/mL to 3.12+/-0.06 and 3.72+/-0.06ng/m (30% and 55% increase) with 50mg/kg and 100mg/kg fruit extract, respectively.[60] One other study using otherwise healthy rats has noted, offhand, an increase in testosterone with both the control drug (Viagra at 0.71mg/kg) and Tribulus fruits at 100mg/kg but does not disclose total increases.[61]

In humans, tribulus terrestris of Bulgarian origin has been shown to have no influence on total testosterone or luteinizing hormone levels when supplemented to healthy men in 200mg a day with a 60% saponin content.[62] It does not seem to influence testosterone levels in females at 450mg (low powered case studies)[63] and when 450mg Tribulus is adminsitered to weight trained men and rugby players for up to 5 weeks fails to note significant increases in testosterone.[53]

It should be noted that based on body surface area conversions[64] an oral dose of 450mg Tribulus equates to 30.94mg/kg in rat studies if we assume a 200lb human. Previous studies with dehydrate fruit extracts of Tribulus[13][61][60] note increases in testosterone with 50-100mg/kg bodyweight in rats, which correlates to a 200lb human ingesting 727mg-1.45g Tribulus fruits.

Some human studies have shown increased testosterone associated with Tribulus supplementation, however they are confounded with coingestion of other nutrients[65][66][67] and one which used 6g of the root extract of tribulus terrestris by itself for 60 days in infertile men noted a trend to increase testosterone (failed to be statistically significant) reaching 16.3%.[68] This is similar to the first study using a formulation known as Tribestan where an increase in testosterone was noted in impotent male rams[69] and suggests that the only role tribulus terrestris may have in increasing testosterone is normalizing testicular damage in impotent subjects.

When looking at animal and human evidence, there is sporadic evidence to suggest an increase in testosterone with this herb in rodents but it has repeatedly failed to increase testosterone in humans. There may be a weak increase in testosterone in infertile men, but this needs to be explored more

8.2. Estrogen

A rat study using female rats and assessing intrinsic estrogenic potential of Tribulus Terrestris found no significant effect of extracts of 11, 42, and 110mg/kg bodyweight where the lowest dose correlates to a 750mg oral intake for a 70kg human.[57]

Currently no evidence to support alterations in estrogen metabolism with supplementation of tribulus terrestris


Edit9. Interactions with other Organ Systems

9.1. Liver

In a rat model of cadmium toxicity, 5mg/kg of an ethanolic extract of tribulus terrestris for six weeks appears to fully normalize changes in oxidative parameters with a potency similar to 75mg/kg vitamin E and attenuates changes in liver protein content and enzymes (potency slightly lesser than vitamin E).[70] This protective effect extends to other minerals such as mercury (6mg/kg of the methanolic fruit extract in mice).[71][72]

In an in vitro tacrine induced toxicity model, the fruits of tribulus terrestris with Tribulusimide D (10-20µM; EC50 13.46+/-0.2µM) and Quercetin-3-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside (20-40µM; EC50 of 7.06+/-0.7 µM) having a potency comparable to Silybin at 100µM; Terrestiamide confirmed inactive[30] and since Tribulusimides A and B have been shown to be hepatoprotective elsewhere[29] it is though this lignanamide class is responsible for hepatoprotection.

Tribulus terrestris appears to have protective effects in the liver, which are more potent than vitamin E when looking at antioxidative changes against mineral toxicity and slightly weaker or comparable when looking at cellular damage and liver enzymes

Anti-inflammatory effects have been noted with the aqueous extract of Tribulus in vitro, where suppression of HepG2 tumor cell growth was noted (and no effect on normal cell growth) and thought to be secondary to increasing IκBα content and reducing NF-kB content, the former of which appears to be through inhibiting the IκBα kinase enzyme (although it did not influence binding of the IKK-β subunit, usually implicated with NF-kB).[73]

9.2. Kidney

In a rat model of cadmium toxicity, 5mg/kg oral tribulus terrestris ethanolic extract over six weeks is able to fully normalize oxidative parameters (TBARS and antioxidant enzymes) and significantly attenuate cadmium buildup in the kidneys, the potency being comparable to 75mg/kg Vitamin E.[70] This protective effect extends to mice, where mercury toxicity with 6mg/kg of a methanolic extract of tribulus terrestris fruits[74][71][72]

In studies on rodents given experimental heavy metal toxicity, tribulus appears to be quite protective with a potency exceeding Vitamin E on a weight basis. This occurs at a concentration low enough that it likely applies to supplementation in humans

A case study of tribulus terrestris overconsumption (2L of a tribulus water product for the treatment of kidney stones) has resulted in nephrotoxicity which was treated in a clinical setting.[75] Although it has been claimed that the particular product in question could have been contaminated with bacteria (the symptoms were similar)[76] a subsequent study in diabetic rats given a hydroalcoholic extract of tribulus terrestris at 50mg/kg (45.84% saponins) reported histological damage to distal tubules and collecting ducts while glomerular function and the renal cortex seemed unaffected.[34] This study also reported improvemetns in diabetic symptoms and an increase in glomerular filtration rate, so it is unsure if this 'toxicity' is relevant to kidney function.[34]

While possible kidney toxicity cannot yet be ruled out, it seems to not be too much of a concern since the case study failed to place causation on the herb and the lone rat intervention also noted improvements in kidney function alongside the 'altered' histology

A 60kDa protein found in tribulus terrestris appears to be able to dissolve calcium oxalate kidney stones in vitro with a potency comparable to cystone.[21] A basic water extract of tribulus terrestris fruits themselves also show this property, with 25-100µg/mL causing 71% inhibition of calcium oxalate nucleation in vitro while showing dose-dependence in preventing stone growth between 25µg/mL (18%) and 1,000µg/mL (170%) with 50-200µg/mL causing 65-70% inhibition.[77] A part of the benefit from tribulus terrestris is thought to be due to flavonoids that inhibit oxalate synthesis, such as Quercetin (Noncompetitive inhibition of glycolate oxidase with an IC50 of 0.5µM) and Kaempferol (Competitive with an IC50 of 0.3µM).[78]

Beyond reducing stone growth, the cell damage induced by calcium oxalate is significantly reduced by 10-50µg/mL of the fruit water extract[77] and the protein itself.[21]

When tribulus terrestris itself is used in rats, it shows dose-dependent protective effects with most potency in the ethanolic extract[79] and when Unex is used (a combination formula of tribulus terrestris and Boerhaavia diffusa) in a rat model of ethylene glycol induced kidney stones, 200mg/kg oral ingestion is able to reduce kidney stone formation with a potency comparable to 750mg/kg cystone (400mg/kg Unex appeared to be slightly less potent).[33]

Tribulus terrestris appears to be able to reduce kidney stone formation in vitro (specifically calcium oxalate stones) which may be due to multiple components. It has shown efficacy in rodents, and when paired with another herb known as Boerhaavia diffusa it appears to be quite potent

In a human study giving 3g of the fruit extract of tribulus terrestris or a water extract thereof, both interventions were effective in increasing urinary output by 200mL daily when supplemented for a month.[45]

The water soluble components in tribulus appear to have diuretic properties in humans

9.3. Testicles

5mg/kg tribulus terrestris in rats is able to attenute cadmium-induced testicular toxicity when preloaded, although with a potency less than 75mg/kg Vitamin E.[80]

Appears to have general antioxidative and mineral chelating effects in the testicles are respectable oral dosages

11mg/kg tribulus terrestris in boars (750mg human equivalent at 70kg bodyweight) appears to increase seminal volume, although 42-110mg/kg was not associated with an increase.[81]

In rats, 50-100mg/kg of the fruits appears to increase sperm count by 25.8-32.2% over 28 days of supplementation.[60]

Testicular weight may be increased with triblus supplementation without any abnormal changes in histological examination.[61]

In humans with oligozoospermia (low sperm count), supplementation of tribulus terrestris at 6g of the root extract daily for two months is able to improve sperm count and motility although the degree of increase was not significantly greater than placebo.[68]

9.4. Prostate

Benign Prostatic Hyperplasia (BPN) is a condition that is not harmful per se but may be indicative of disease pathology leading to more serious diseases such as Prostate Cancer and may be associated with impairment of a male's quality of life or interfering with urinary flow rates. The current most promising over-the-counter remedies for BPN are Stinging Nettle with some efficacy seen with Saw Palmetto.

Tribulus has been investigated for its benefits on BPN, although this study used a combination supplement of Tribulus Terrestris and Murraya koenigii.[1] Although only 12 weeks in length, the combination supplement was able to reduce IPSS symptoms from a score of 17 down to 9, which was not significantly different than the efficacy of 400mcg tamsulosin reducing IPSS symptoms from 14 to 8; the herbal combination reduced prostatic volume from 33.5mL to 31.6mL (5.6% reduction) and was statistically significant while the 3.4% reduction seen with tamsulosin did not reach significance.[1] This study was criticized for its usage of generic tamsulosin (and thus no control for active amounts of tamsulosin) paired with a lack of placebo group.[82]

Another possible confound to the above research is that IPPS symptoms include urinary flow, as Benign Prostatic Hyperplasia may sometimes reduce flow rate. Tribulus itself appears to possess diuretic properties, although this study used a large dose of Tribulus where 5g/kg in Guinea pigs was as effective as furosemide.[83]

Possibly beneficial for benign prostatic hyperplasia, but evidence is limited and no mechanisms are known


Edit10. Interactions with Sexuality

10.1. Libido

A study in fertile boars with combination therapy of tribulus terrestris, Eurycoma Longifolia Jack and Leuzea carthamoides noted that 11mg/kg oral administration (correlating to a 750mg oral dose for a 70kg human) increased mounting behaviour by 20% and increase seminal volume; higher doses (42mg/kg and 110mg/kg) were unaffective.[81]

Various other animal models confirm aphrodisiac effects, including the lyophilized extract dried fruits at 50mg/kg and 100mg/kg in sexually sluggish male rats which noted dose-dependent increases in all parameters of sexuality and penile function increasing with prolonged administration.[60] Intake of tribulus at lower doses of 2.5, 5, and 10mg/kg for a period of 8 weeks was associated with decreases in the latency of mounting, intromission, and ejaculation while improving mounting and intromission frequency; with 5mg/kg and 10mg/kg being of equal potency[36] and in castrated rats Tribulus appears to be slightly less effective at 5mg/kg than were biweekly injections of testosterone cypionate at inducing aphrodisia, but both groups were lesser than an uncastrated control[84] while another study comparing Tribulus Terrestris against Sildenafil Citrate (Viagra) in sexually normal rats at doses of 100mg/kg bodyweight and 0.71mg/kg, respectively, found that there were no significant differences between both groups, as both were more effective than control at inducing aphrodisia with similar efficacy.[61]

In men with low sperm count, supplementation of 6g tribulus root is associated with a significantly greater improvement in sexual health (assessed by survey) by 49.38% which was greater than placebo (27.80%).[68]

10.2. Erectile Properties

In the rabbit corpus cavernosum in vitro, tribulus terrestris fruit extract (lyophilized water extract) at 0.25- 4mg/mL is able to induce relaxation in precontracted tissue in a concentration dependent manner and is independent of Nitric Oxide metabolism (not inhibited by L-NAME or methylene blue), prostaglandin metabolism (indomethacin ineffective), and independent of potassium channels (4-AP and glibenclamide ineffectve).[85] Changes in cAMP and cGMP could also not explain the observed effects.[85]

Elsewhere, a mixture of tribulus terrestris and cornus officinalis (the latter working via nitric oxide metabolism) has noted that tribulus alone was more effective than all mixtures of the two herbs and that the mixture was no more proerectile following oral ingestion than tribulus alone.[86]

An increase in erectile properties have been noted in rabbits given 2.5-100mg/kg of a tribulus fruit extract for one month[85] and this dosage range has been noted to enhance the efficacy of pro-erectile signalling molecules (acetylcholine or electrical stimulation) without affecting anti-erectile signalling molecules.[87] Protodioscin by itself in ineffective[87] and this same dosage range in rats appears to enhance the intracavernous pressure (ICP) of the penis by 17-43% (most efficacy at 5mg/kg) during erections.[36]

Tribulus terrestris appears to relax penile tissue in vitro and enhances erections after oral ingestion, but the difference in dosages (quite high in vitro yet low oral doses) suggest differing mechanisms of action. The mechanism by which tribulus increases erections is not known, but may be related to central androgen receptors

In persons with low sperm count given 6g of tribulus root daily for two months (where almost half of participants reported erectile problems) there was significant improvements in the loss of erection (6.03%), rigidity (9.41%), premature ejaculation (6.12%), and lack of orgasm (9.76%).[68]

Increases in erectile properties and sexual satisfaction in men has been improved with the standard oses of tribulus supplementation


Edit11. Interactions with Aesthetics

11.1. Hair

Tribulus terrestris seems to also be able to stimulate Melanocyte-stimulating hormone when orally administered at a large dose (1g/kg for humans), which increased the rate of MSH detection in hair follicles from 18.75% in control to 75% in the Tribulus group.[88] A later in vitro study demonstrated that a result of Melanocyte-Stimulating Hormone, the actual proliferation of melanocytes, appears to be concentration dependent with 0.5mg/mL actually inhibiting proliferation and 1.5mg/mL inducing proliferation.[89] These effects on melanocyte differentiation were also correlated with tyrosinase activity, suggesting that high doses of Tribulus may have the ability to induce hair pigmentation.

May have the ability to induce coloration of hair follicles at high concentrations, but results are too preliminary to implicate any practical guides

11.2. Skin

Tribulus terrestris fruits at a 70% ethanolic extract at 50μg/mL is able to prevent UVB-induced cell death in skin cells, reducing 87.9% apoptosis to 25.5% associated with inhibiting NF-kB translocation[90] and augmenting the UVB-induced increase in nucleotide excision repair genes (XPA and XPC, two major DNA repair genes[91]) which were not increased in control cells nor in cancerous skin cells (squamous cell carcinoma).[90]

Appears to increase DNA repair in skin cells that are exposed to radiation, and this does not occur in cancerous cells


Edit12. Safety and Toxicology

12.1. General

A 28-day oral toxicology test of 500mg/kg lyophilized dry fruit extract in rats (80mg/kg human dose) failed to establish any toxic signs[60] and another LD50 study found that the LD50 of a basic lyophilized extract of Tribulus (aerial parts and root) as assessed by intraperitoneal injections was 813mg/kg and the 95% condfidence interval calculated to be 739-894mg/kg,[92] suggesting that a lethal dose of Tribulus injections in humans would be (based on body surface area conversions) 118-143mg/kg. Due to unknown bioavailability, it cannot be estalished what a toxic oral dose would be.

Doses typically used for supplementation do not appear to be lethal or harmful

12.2. Case Studies

One case study noted gynecomastia and reduced testosterone associated with Tribulus terrestris supplementation, amidst other factors.[93] This study involved a 21-year old man with a history of breast swelling whom used cigarettes (2 daily) and drank Alcohol (10 units weekly) who reported development of a benign nodule in the left breast that coincided with consumption of a Tribulus product (unnamed). Causation could not be placed on the product.[93]

Standard assortment of case studies, with little evidence to outright blame Tribulus for the effects caused

12.3. Kidneys

A case study of tribulus terrestris overconsumption (2L of a tribulus water product for the treatment of kidney stones) has resulted in nephrotoxicity which was treated in a clinical setting[75] (which is thought to be confounded with the possibility of microbial contamination as the symptoms were similar[76]) and a subsequent study in diabetic rats given a hydroalcoholic extract of tribulus terrestris at 50mg/kg (45.84% saponins) reported histological damage to distal tubules and collecting ducts while glomerular function and the renal cortex seemed unaffected.[34] Additionally serum creatinine, cystatin c, and beta 2 microglobulin were reduced in these rats and protein/albumin normalized which suggested an increase in glomerular filtration rate (improvement in kidney function).[34]

There are well established instances of tribulus terrestris induced neurotoxicity and renal toxicity in ruminant animals (known as Geeldikkop and Staggers)[94][95] although this does not appear to extend to humans.

There is limited evidence to support an increase in kidney toxicity with tribulus terrestris. While the case study did pretty much note causation to the product it is possible that it was contaminated, and the rat study noting renal toxicity had quite mixed results and it is difficult to reach a conclusion at this point in time

References

  1. Comparison of Murraya koenigii- and Tribulus terrestris-based oral formulation versus tamsulosin in the treatment of benign prostatic hyperplasia in men aged >50 years: a double-blind, double-dummy, randomized controlled trial
  2. Iacono F, et al. Observational study: daily treatment with a new compound "Tradamixina" plus serenoa repens for two months improved the lower urinary tract symptoms. BMC Surg. (2012)
  3. Iacono F, et al. Sexual asthenia: Tradamixina versus Tadalafil 5 mg daily. BMC Surg. (2012)
  4. Sharma J, et al. The treatment of jaundice with medicinal plants in indigenous communities of the Sub-Himalayan region of Uttarakhand, India. J Ethnopharmacol. (2012)
  5. Xu YJ, et al. Two new furostanol saponins from Tribulus terrestris. J Asian Nat Prod Res. (2010)
  6. A comparative phylogenetic analysis of medicinal plant Tribulus terrestris in Northwest India revealed by RAPD and ISSR markers
  7. Mutheeswaran S, et al. Documentation and quantitative analysis of the local knowledge on medicinal plants among traditional Siddha healers in Virudhunagar district of Tamil Nadu, India. J Ethnopharmacol. (2011)
  8. Deole YS, et al. Evaluation of anti-depressant and anxiolytic activity of Rasayana Ghana Tablet (A compound Ayurvedic formulation) in albino mice. Ayu. (2011)
  9. Kevalia J, Patel B. Identification of fruits of Tribulus terrestris Linn. and Pedalium murex Linn.: A pharmacognostical approach. Ayu. (2011)
  10. Olas B, et al. Evaluation of polyphenolic fraction isolated from aerial parts of Tribulus pterocarpus on biological properties of blood platelets in vitro. Platelets. (2012)
  11. Flavonoid glycosides of Tribulus pentandrus and T. Terrestris
  12. Temraz A, et al. Steroidal saponins from the aerial parts of Tribulus alatus Del. Phytochemistry. (2006)
  13. El-Tantawy WH, Temraz A, El-Gindi OD. Free serum testosterone level in male rats treated with Tribulus alatus extracts. Int Braz J Urol. (2007)
  14. Dinchev D, et al. Distribution of steroidal saponins in Tribulus terrestris from different geographical regions. Phytochemistry. (2008)
  15. AN IMPROVED METHOD FOR THE EXTRACTION AND QUANTITATION OF DIOSGENIN IN TRIBULUS TERRESTRIS L
  16. Zhang S, Li H, Yang SJ. Tribulosin suppresses apoptosis via PKC epsilon and ERK1/2 signaling pathway during hypoxia/reoxygenation in neonatal rat ventricular cardiac myocytes. J Asian Nat Prod Res. (2011)
  17. Sun W, Li H, Yang SJ. A triterpene saponin from Tribulus terrestris attenuates apoptosis in cardiocyte via activating PKC signalling transduction pathway. J Asian Nat Prod Res. (2008)
  18. Conrad J, et al. A novel furostanol saponin from Tribulus terrestris of Bulgarian origin. Fitoterapia. (2004)
  19. Su L, et al. Steroidal saponins from Tribulus terrestris. Steroids. (2009)
  20. Hammoda HM, et al. Chemical constituents from Tribulus terrestris and screening of their antioxidant activity. Phytochemistry. (2013)
  21. Aggarwal A, et al. A novel antilithiatic protein from Tribulus terrestris having cytoprotective potency. Protein Pept Lett. (2012)
  22. Gacche RN, Dhole NA. Profile of aldose reductase inhibition, anti-cataract and free radical scavenging activity of selected medicinal plants: an attempt to standardize the botanicals for amelioration of diabetes complications. Food Chem Toxicol. (2011)
  23. Chen G, et al. New steroidal glycosides from Tribulus terrestris L. J Asian Nat Prod Res. (2012)
  24. Coates TJ, Lo B. Counseling patients seropositive for human immunodeficiency virus. An approach for medical practice. West J Med. (1990)
  25. Wang J, Zu X, Jiang Y. Five furostanol saponins from fruits of Tribulus terrestris and their cytotoxic activities. Nat Prod Res. (2009)
  26. Xu T, et al. Two new furostanol saponins from Tribulus terrestris L. Fitoterapia. (2009)
  27. Liu T, et al. Two new steroidal saponins from Tribulus terrestris L. J Asian Nat Prod Res. (2010)
  28. Liu T, et al. New pregnane and steroidal glycosides from Tribulus terrestris L. J Asian Nat Prod Res. (2010)
  29. Li JX, et al. Tribulusamide A and B, new hepatoprotective lignanamides from the fruits of Tribulus terrestris: indications of cytoprotective activity in murine hepatocyte culture. Planta Med. (1998)
  30. Byun E, et al. Tribuli fructus constituents protect against tacrine-induced cytotoxicity in HepG2 cells. Arch Pharm Res. (2010)
  31. Zhang X, et al. A new feruloyl amide derivative from the fruits of Tribulus terrestris. Nat Prod Res. (2012)
  32. Kostova I, et al. Two new sulfated furostanol saponins from Tribulus terrestris. Z Naturforsch C. (2002)
  33. Jarald EE, et al. Effect of Unex on ethylene glycol-induced urolithiasis in rats. Indian J Pharmacol. (2011)
  34. Gandhi S, Srinivasan BP, Akarte AS. Potential nephrotoxic effects produced by steroidal saponins from hydro alcoholic extract of Tribulus terrestris in STZ-induced diabetic rats. Toxicol Mech Methods. (2013)
  35. Orhan I, et al. Acetylcholinesterase and butyrylcholinesterase inhibitory activity of some Turkish medicinal plants. J Ethnopharmacol. (2004)
  36. Gauthaman K, Ganesan AP, Prasad RN. Sexual effects of puncturevine (Tribulus terrestris) extract (protodioscin): an evaluation using a rat model. J Altern Complement Med. (2003)
  37. Wang Z, et al. Effect of tribulus terrestris saponins on behavior and neuroendocrine in chronic mild stress depression rats. J Tradit Chin Med. (2013)
  38. Heidari MR, et al. The analgesic effect of Tribulus terrestris extract and comparison of gastric ulcerogenicity of the extract with indomethacine in animal experiments. Ann N Y Acad Sci. (2007)
  39. Ranjithkumar R, et al. Standardized Aqueous Tribulus terristris (Nerunjil) Extract Attenuates Hyperalgesia in Experimentally Induced Diabetic Neuropathic Pain Model: Role of Oxidative Stress and Inflammatory Mediators. Phytother Res. (2012)
  40. Mazaro-Costa R, et al. Medicinal plants as alternative treatments for female sexual dysfunction: utopian vision or possible treatment in climacteric women. J Sex Med. (2010)
  41. Wang SS, et al. Mechanisms of gross saponins of Tribulus terrestris via activating PKCepsilon against myocardial apoptosis induced by oxidative stress. Yao Xue Xue Bao. (2009)
  42. Zhang S, Li H, Yang SJ. Tribulosin protects rat hearts from ischemia/reperfusion injury. Acta Pharmacol Sin. (2010)
  43. Dong S, et al. Post-conditioning protects cardiomyocytes from apoptosis via PKC(epsilon)-interacting with calcium-sensing receptors to inhibit endo(sarco)plasmic reticulum-mitochondria crosstalk. Mol Cell Biochem. (2010)
  44. Zhang J, et al. ERK-MAPK signaling opposes rho-kinase to reduce cardiomyocyte apoptosis in heart ischemic preconditioning. Mol Med. (2010)
  45. Murthy AR, Dubey SD, Tripathi K. Anti-hypertensive effect of Gokshura (Tribulus terrestris Linn.) - A clinical study. Anc Sci Life. (2000)
  46. Somanadhan B, et al. An ethnopharmacological survey for potential angiotensin converting enzyme inhibitors from Indian medicinal plants. J Ethnopharmacol. (1999)
  47. Sharifi AM, Darabi R, Akbarloo N. Study of antihypertensive mechanism of Tribulus terrestris in 2K1C hypertensive rats: role of tissue ACE activity. Life Sci. (2003)
  48. Sajid TM, Rashid S, Saeed SA. Inhibition of adrenaline-induced aggregation of human platelets by Pakistani medicinal plants. Pak J Pharm Sci. (1991)
  49. Ponnusamy S, et al. Evaluation of traditional Indian antidiabetic medicinal plants for human pancreatic amylase inhibitory effect in vitro. Evid Based Complement Alternat Med. (2011)
  50. Zhang SJ, Qu WJ, Zhong SY. Inhibitory effects of saponins from Tribulus terrestris on alpha-glucosidase in small intestines of rats. Zhongguo Zhong Yao Za Zhi. (2006)
  51. Amin A, et al. The protective effect of Tribulus terrestris in diabetes. Ann N Y Acad Sci. (2006)
  52. Antonio J, et al. The effects of Tribulus terrestris on body composition and exercise performance in resistance-trained males. Int J Sport Nutr Exerc Metab. (2000)
  53. Rogerson S, et al. The effect of five weeks of Tribulus terrestris supplementation on muscle strength and body composition during preseason training in elite rugby league players. J Strength Cond Res. (2007)
  54. Hong CH, et al. Evaluation of natural products on inhibition of inducible cyclooxygenase (COX-2) and nitric oxide synthase (iNOS) in cultured mouse macrophage cells. J Ethnopharmacol. (2002)
  55. Gauthaman K, Adaikan PG. Effect of Tribulus terrestris on nicotinamide adenine dinucleotide phosphate-diaphorase activity and androgen receptors in rat brain. J Ethnopharmacol. (2005)
  56. Vincent SR, Kimura H. Histochemical mapping of nitric oxide synthase in the rat brain. Neuroscience. (1992)
  57. Martino-Andrade AJ, et al. Effects of Tribulus terrestris on endocrine sensitive organs in male and female Wistar rats. J Ethnopharmacol. (2010)
  58. Nahata A, Dixit VK. Evaluation of 5α-reductase inhibitory activity of certain herbs useful as antiandrogens. Andrologia. (2013)
  59. Gauthaman K, Ganesan AP. The hormonal effects of Tribulus terrestris and its role in the management of male erectile dysfunction--an evaluation using primates, rabbit and rat. Phytomedicine. (2008)
  60. Singh S, Nair V, Gupta YK. Evaluation of the aphrodisiac activity of Tribulus terrestris Linn. in sexually sluggish male albino rats. J Pharmacol Pharmacother. (2012)
  61. Aphrodisiac activity of Tribulus terrestris Linn. in experimental models in rats
  62. Neychev VK, Mitev VI. The aphrodisiac herb Tribulus terrestris does not influence the androgen production in young men. J Ethnopharmacol. (2005)
  63. Saudan C, et al. Short term impact of Tribulus terrestris intake on doping control analysis of endogenous steroids. Forensic Sci Int. (2008)
  64. Guidance for Industry Estimating the Maximum Safe Starting Dose in Initial Clinical Trials for Therapeutics in Adult Healthy Volunteers
  65. Brown GA, et al. Effects of anabolic precursors on serum testosterone concentrations and adaptations to resistance training in young men. Int J Sport Nutr Exerc Metab. (2000)
  66. Brown GA, et al. Effects of androstenedione-herbal supplementation on serum sex hormone concentrations in 30- to 59-year-old men. Int J Vitam Nutr Res. (2001)
  67. Brown GA, et al. Endocrine and lipid responses to chronic androstenediol-herbal supplementation in 30 to 58 year old men. J Am Coll Nutr. (2001)
  68. Sellandi TM, Thakar AB, Baghel MS. Clinical study of Tribulus terrestris Linn. in Oligozoospermia: A double blind study. Ayu. (2012)
  69. Dimitrov M, Georgiev P, Vitanov S. Use of tribestan on rams with sexual disorders. Vet Med Nauki. (1987)
  70. Lakshmi GD, et al. Protective effect of Tribulus terrestris linn on liver and kidney in cadmium intoxicated rats. Indian J Exp Biol. (2012)
  71. Jagadeesan G, Kavitha AV. Recovery of phosphatase and transaminase activity of mercury intoxicated Mus musculus (Linn.) liver tissue by Tribulus terrestris (Linn.) (Zygophyllaceae) extract. Trop Biomed. (2006)
  72. Jagadeesan G, Kavitha AV, Subashini J. FT-IR Study of the influence of Tribulus terrestris on Mercury intoxicated mice, Mus musculus liver. Trop Biomed. (2005)
  73. Kim HJ, et al. Aqueous extract of Tribulus terrestris Linn induces cell growth arrest and apoptosis by down-regulating NF-κB signaling in liver cancer cells. J Ethnopharmacol. (2011)
  74. Kavitha AV, Jagadeesan G. Role of Tribulus terrestris (Linn.) (Zygophyllacea) against mercuric chloride induced nephrotoxicity in mice, Mus musculus (Linn.). J Environ Biol. (2006)
  75. Talasaz AH, et al. Tribulus terrestris-induced severe nephrotoxicity in a young healthy male. Nephrol Dial Transplant. (2010)
  76. Schmidt M, Thomsen M, Bone K. Tribulus terrestis-induced nephrotoxicity. Nephrol Dial Transplant. (2011)
  77. Aggarwal A, et al. Diminution of oxalate induced renal tubular epithelial cell injury and inhibition of calcium oxalate crystallization in vitro by aqueous extract of Tribulus terrestris. Int Braz J Urol. (2010)
  78. Shirfule AL, Sangamwar AT, Khobragade CN. Exploring glycolate oxidase (GOX) as an antiurolithic drug target: molecular modeling and in vitro inhibitor study. Int J Biol Macromol. (2011)
  79. Anand R, et al. Activity of certain fractions of Tribulus terrestris fruits against experimentally induced urolithiasis in rats. Indian J Exp Biol. (1994)
  80. Rajendar B, et al. Protective effect of an aphrodisiac herb Tribulus terrestris Linn on cadmium-induced testicular damage. Indian J Pharmacol. (2011)
  81. Frydrychová S, et al. Effects of herbal preparation on libido and semen quality in boars. Reprod Domest Anim. (2011)
  82. Kaplan SA. Re: Comparison of Murraya koenigii- and Tribulus terrestris-Based Oral Formulation Versus Tamsulosin in the Treatment of Benign Prostatic Hyperplasia in Men Aged >50 Years: A Double-Blind, Double-Dummy, Randomized Controlled Trial. J Urol. (2012)
  83. Al-Ali M, et al. Tribulus terrestris: preliminary study of its diuretic and contractile effects and comparison with Zea mays. J Ethnopharmacol. (2003)
  84. Gauthaman K, Adaikan PG, Prasad RN. Aphrodisiac properties of Tribulus Terrestris extract (Protodioscin) in normal and castrated rats. Life Sci. (2002)
  85. Do J, et al. Effects and Mechanism of Action of a Tribulus terrestris Extract on Penile Erection. Korean J Urol. (2013)
  86. Kam SC, et al. In Vivo and in Vitro Animal Investigation of the Effect of a Mixture of Herbal Extracts from Tribulus terrestris and Cornus officinalis on Penile Erection. J Sex Med. (2012)
  87. Adaikan PG, et al. Proerectile pharmacological effects of Tribulus terrestris extract on the rabbit corpus cavernosum. Ann Acad Med Singapore. (2000)
  88. Yang L, et al. Effect of Tribulus terrestris extract on melanocyte-stimulating hormone expression in mouse hair follicles. Nan Fang Yi Ke Da Xue Xue Bao. (2006)
  89. Deng Y, Yang L, An SL. Effect of Tribulus terrestris L decoction of different concentrations on tyrosinase activity and the proliferation of melanocytes. Di Yi Jun Yi Da Xue Xue Bao. (2002)
  90. Sisto M, et al. Saponins from Tribulus terrestris L. protect human keratinocytes from UVB-induced damage. J Photochem Photobiol B. (2012)
  91. Sancar A, et al. Molecular mechanisms of mammalian DNA repair and the DNA damage checkpoints. Annu Rev Biochem. (2004)
  92. Arcasoy HB, et al. Effect of Tribulus terrestris L. saponin mixture on some smooth muscle preparations: a preliminary study. Boll Chim Farm. (1998)
  93. Jameel JK, et al. Gynaecomastia and the plant product "Tribulis terrestris". Breast. (2004)
  94. Aslani MR, et al. Experimental Tribulus terrestris poisoning in sheep: clinical, laboratory and pathological findings. Vet Res Commun. (2003)
  95. Bourke CA. Hepatopathy in sheep associated with Tribulus terrestris. Aust Vet J. (1983)

(Common misspellings for Tribulus terrestris include territis, terrestis, terestris, tibulus, tribuls)

(Common phrases used by users for this page include tribulus for 21 year old, the best time to take on tribulus terrestris, side effects of matrix tribulus, on tribulus review, if you take tribulus do you need to take anti estrogen, "sexual effects of puncturevine (tribulus terrestris) extract (protodioscin): an evaluation using a rat model)

(Users who contributed to this page include Insamity, )