Tetradecyl Thioacetic Acid

Technically an Omega-3 fatty acid, TTA is a non-metabolizable fatty acid that cannot be used for energy and may be able to burn fat via mechanisms similar to Conjugated Linoleic Acid. Lacking studies in humans at the moment, TTA appears to be a promising future candidate for fat loss and health.

This page features 31 unique references to scientific papers.


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Tetradecyl Thioacetic Acid, otherwise known as TTA, is what is known as a PPAR-alpha activator. It is actually an omega-3 fatty acid, but has a sulfur group at the omega-3 position; because of this addition it cannot be burnt for energy and thus has no relevant caloric value to humans.

PPARa activation can be seen as protecting the body from excess fats (similar to PPARy activation). PPARa tends to clear fats from the blood into muscle or liver cells, and encourage them to be burnt for energy in these locations (by comparison, PPARy makes new fat cells for fats to reside in which minimizes their potential toxicity).

The clearing of fat from the blood causes a drop in lipoproteins and a lowering of LDL cholesterol, and the burning of fats causes either fat burning or reduced fat gain. TTA can also decrease blood pressure and exert an anti-oxidant effect, the four mechanisms making it a cardioprotective compound.

It is commonly used with the appetite suppressant Oleoylethanolamide as a fat-burning combination.

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Also Known As

TTA


Things to Note

  • Tetradecyl Thioacetic Acid (TTA) is non-stimulatory
  • The taste and texture of TTA powder has been described as similar to styrofoam
  • TTA is fat soluble and should be taken with meals

Is a Form of


Goes Well With


Caution Notice

Examine.com Medical Disclaimer

Not much evidence currently exists in human, but the standard dose appears to be 1g of TTA daily taken in divided dosages with meals.


The Human Effect Matrix looks at human studies (excluding animal/petri-dish studies) to tell you what effect Tetradecyl Thioacetic Acid has in your body, and how strong these effects are.
GradeLevel of Evidence
ARobust research conducted with repeated double blind clinical trials
BMultiple studies where at least two are double-blind and placebo controlled
CSingle double blind study or multiple cohort studies
DUncontrolled or observational studies only
Level of Evidence
EffectChange
Magnitude of Effect Size
Scientific ConsensusComments
CTotal Cholesterol

Minor

Possible reductions in total cholesterol with supplementation of TTA

CTriglycerides

Notable

The one trial using TTA to reduce triglycerides noted a reduction of around 15%, which is notable and requires replication

CTNF-Alpha

Minor

A decrease in serum levels of TNF-a has been noted with supplemental TTA

CCell Adhesion Factors

Minor

Expression of VCAM-1 has been noted to be decreased

DLDL-C

Minor

Possible reductions in LDL cholesterol seen with TTA consumption

DLiver Enzymes

No significant influence on liver enzymes in preliminary testing for possible toxicity

DUric Acid

No significant alterations in serum uric acid seen with supplemental TTA

DC-Reactive Protein

No significant influence on C-Reactive Protein levels

DHbA1c

No significant alterations in HbA1c concentrations

DBlood Pressure

Minor

A slight reduction in blood pressure has been noted in dyslipidemic obese men with TTA supplementation

DWeight

Although increased mitochondrial fat oxidation was created for underlying the reductions in lipids and blood pressure, no significant influence on body weight was noted... show

DHDL-C

Minor

An increase in HDL cholesterol has been noted with TTA consumption

DBlood Glucose
DKidney function
DRed Blood Cell Count
DWhite Blood Cell Count
DHeart Rate
DThyroid-Stimulating Hormone
DSerum T4
DViral Load
DApolipoprotein B
DInsulin Sensitivity

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Table of Contents:


Edit1. Sources and Structure

1.1. Structure

Tetradecyl thioacetic acid (TTA) is a nondietary fatty acid which belongs to the omega-3 class, but is also considered a thia fatty acid as it possesses a sulfur group in the fatty acid chain. It does not have the ability to be β-oxidized due to this, and does not confer to bodily energy requirements.


Edit2. Molecular Targets

2.1. PPARs

In isolated human liver cells 10-30µM TTA is able activate PPARα and PPARδ with preference for PPARα activation,[1] while PPARγ was not significantly influenced until a concentration of 75µM (and even then, it was only 60% potency of the reference drug rosiglitazone).[1] PPARδ activation has also been noted in myotubes by increasing fatty acid oxidation with a similar potency to the research standard GW501516[1] increasing the downstream targets of CPT-1 (almost 2-fold) and CD36/FAT (almost 3-fold) relative to control,[1] and activation of PPARδ is thought to also be a target of fat metabolism in liver cells as deleting PPARα is not sufficient to ablate the efficacy of TTA while increasing the protein content of PPARδ increases the in vitro efficacy of TTA in liver cells.[2]

Due to oral supplementation of TTA in humans either significantly[1][3] or nonsignificantly[4] reducing total lipid levels in the blood (thought to reflect baseline lipid concentrations), it is thought that the above mechanisms are relevant to supplementation of TTA.

Tetradecylthioacetic acid (TTA) appears to be a ligand and activator for PPARs, specifically the alpha and delta subsets. When activated, these receptors are thought to positively mediate the oxidation of fatty acids in both liver and muscle cells


Edit3. Pharmacology

3.1. Absorption

Tetradecyl thioacetic acid (TTA) has been noted to reduce the secretion of triglycerides rich lipoproteins[5] from an intestinal cell line known as Caco-2 when coincubated with fatty acids.[5] Unlike in liver cells where the reduction in triglyceride secretion appears to be secondary to increasing fatty acid oxidation,[6][6] the two phenomena appear to be independent of one another in Caco-2 cells.[5]

TTA itself is absorbed into Caco-2 cells[5][7] and despite the reduced secretion of triglyceride rich lipoproteins with TTA relative to oleic acid they have comparable rates of incorporation; there does not appear to be marked accumulation of TTA-enriched lipoproteins within the cell, thought to be due to TTA more readily forming complexes with phospholipids.[5] TTA is known to be ultimately absorbed from the intestines due to it being detected in serum following supplementation, although the exact bioavailability is unknown.

3.2. Serum

Following oral ingestion, Tetradecyl thioacetic acid (TTA) appears to have a lag time of approximately 90 minutes where minimal to no TTA is detected in the blood followed by rapid detection in the blood.[4] Following this TTA appears to be detectable in the blood at median peak values (estimated Cmax values) of 2.9mg/L (200mg), 11.5mg/L (600mg), and 11mg/L (1,000mg) at variable mean Tmax values of 2.5-4.5 hours following oral administration; all values had a high range of individual variability.[4]

TTA can be detected in the blood following a single dose, but appears to have a lag time before absorption and a fairly variable peak concentration and time to reach peak concentration between subjects.

Chronic loading for seven days at three doses (200mg, 600mg, and 1,000mg) all demonstrate increases in serum TTA with subsequent doses, suggesting a loading effect due to incomplete drug elimination within 24 hours.[4] A dose-dependent relationship is more apparent after a week, where the doses reach circulating plasma concentrations of 4mg/L, 9mg/L, and 14mg/L in the 200mg, 600mg, and 1,000mg groups respectively.[4]

When looking at total blood lipids, TTA appears to increase from nonexistent up to 0.44+/-0.03% of blood lipids following a month of 1,000mg supplementation[3] whereas the major metabolite of TTA 1n-8 comprises 0.14+/-0.03% of total blood lipids.[3]

Chronic loading is similar to acute loading, although a dose-dependent effect is more apparent. The maximum concentration seem with acute usage at 1,000mg does not appear to be increased further with chronic usage.

3.3. Distribution

TTA has a volume of distribution ranging from 52.3 to 84.3 L based on oral dose and interindividual differences, which suggest binding to the lipid compartment of the blood.[4]

3.4. Metabolism

In serum with chronic loading of 600mg or 1,000mg, a delta-9-desaturase derivative of TTA has been detected (TTA 1n-8) suggesting that it is metabolized via this enzyme in humans.[4]

Due to being a sulfated fatty acid, TTA cannot be metabolized by the process of β-oxidation for utilization as energy.[8][9]

3.5. Elimination

After one week of supplementing tetradecyl thioacetic acid (TTA) in the range of 200-1000mg, TTA was still found in the body by day 14 (one week of cessation) but was not detectable after one month after the start of the study (three weeks of cessation).[4] Approximately 80% of circulating TTA is eliminated by 1 week of cessation, returning to baseline 3 weeks after cessation.[4]

Rate of clearance was estimated to be 4.1-5.6L per hour based on oral doses of 200,600, and 1000mg.[4] The estimated half-life of TTA following oral administration in humans is between 8.9 and 14 hours.[4]

TTA appears to be slowly metabolized and has a half-life reaching about half a day, indicating that a single oral dose would influence the body for over a 24 hour period. TTA may still be detectable in the blood following a week cessation from the supplement, but is no longer detectable after three weeks.


Edit4. Cardiovascular Health

4.1. Absorption

Tetradecyl thioacetic acid (TTA) is known to attenuate the stimulation of triglyceride-rich lipoprotein secretion from other fatty acids such as oleic acid when coincubated in vitro in intestinal cells[5] and in liver cells,[6] suggesting that there may be an inhibitory effect on the release of circulating fatty acids from these two organs that may contribute to the hypolipidemic effects observed with TTA ingestion.

There is a possible inhibitory effect on complete fatty acid absorption associated with TTA supplementation, although this is not related to an inhibition of the absorption of fatty acids but reducing their release into the blood via typical routes. Practical significance of this mechanism to oral supplementation is not yet known

4.2. Cardiac Tissue

In rats subject to a controlled diet containing 0.375% TTA over the course of 50 weeks, there appears to be a modification in the fatty acids of cardiac tissue with a decrease in saturated fat and aradchidonic acid content with an increase in Fish Oil fatty acids, and this effect was additive with fish oil supplementation (10.4% of diet).[10]

May increase fish oil fatty acid content of the heart independent of fish oil supplementation

4.3. Atherosclerosis

The scavenger receptor A (SRA) is a receptor that is thought to play antiinflammatory (via reducing TNF-α[11]) and antiatherosclerotic roles (by reducing LDL cholesterol levels[12]).

One study in humans with HIV given TTA supplementation for one month at the dose of 1,000mg found that supplementation was associated with an increase in scavenger receptor A (SRA) with supplementation without any influence on LDL receptor mRNA (in tested PMBCs).[13] The authors noted that, in mice, supplementation caused an increase in hepatic SRA mRNA content within five days of supplementation although these mice also experienced increases in LDL receptor mRNA in the liver[13] and due to an inverse relation between total cholesterol levels and TNF-α concentrations it was thought that the SRA played a significant role.

4.4. Lipoproteins and Triglycerides

In addition to the above mechanisms (reduction of fatty acids in the blood, reduced fat mass) TTA can alleviate the decrease in insulin sensitivity from a fat promoting diet.[14] and has antioxidant abilities.[15] Thus TTA may be a potent cardioprotective agent, and appears to be more protective in the metabolically impaired.[16]

In non-metabolically impaired hearts, TTA may suppress overall cardiac output via the increase in fatty acid oxidation causing a decrease in glucose oxidation.[17]


Edit5. Interactions with Glucose Metabolism

5.1. Glycation

A study in persons with HIV on anti-viral therapy who were then given 1,000mg of TTA supplementation daily over the course of one month failed to find any modifications in HbA1c concentrations relative to baseline.[13]

5.2. Insulin sensitivity

In persons with HIV on antiviral therapy given an additional 1,000mg TTA, supplementation failed to significantly alter insulin sensitivity relative to baseline.[13]


Edit6. Body Fat and Obesity

6.1. Interventions

When supplemented to an obesity-inducing and insulin resistance promoting diet, TTA shows promise in negating a fair bit of the gain in mass and completely ameliorating the onset of peripheral insulin resistance.[18] It seems to do this effect via increasing uptake of fats from the blood into tissues that express PPARa (muscle and liver mostly, some kidney) and then to oxidize the fats.[18][19] With superloading of TTA (at 200mg/kg bodyweight) rats experienced weight loss despite eating a greater amount of calories.[20]

No significant effects of TTA at 1g daily over 28 days in dyslipidemic diabetic men has been noted where weight remained stable at 96.3+/-15.9kg,[1] and supplementation of the same dose of TTA for 28 days in HIV positive (normal weight) persons on a controlled diet (to reduce blood lipids and maintain weight) has failed to significantly reduce body weight relative to baseline.[13]


Edit7. Inflammation and Immunology

7.1. Virology

One study investigating the influence of TTA on persons with HIV currently undergoing anti-viral therapy found a decrease of the circulating inflammatory cytokine TNF-α yet no influence on viral loads in immune cells.[13]

The lone study of TTA in persons with HIV noted that it was safe to use, but failed to find any modification (beneficial or negative) to the viral loads in these subjects


Edit8. Interactions with Hormones

8.1. Thyroid Hormones

One study assessing the safety and pharmacology of supplemental TTA noted that one subject in the 1,000mg group experienced an increase in free T4 and reduction in TSH, although it was not deemed clinically relevant and over the whole group there were no other noticeable changes in thyroid function.[4]


Edit9. Interactions with Organ Systems

9.1. Liver

TTA appears to induce mitochondrial β-oxidation in liver cells (mice) via activation of PPARα[21] and in cultured cells treated with TTA a decrease in triglycerides is noted;[6] this is thought to underlie both antiobese and lipid lowering properties of TTA.[22] Beyond activation of PPARα, PPARδ[1] may also be a target as are the stress factors mTOR and ERK1/2.[23]

Increasing fatty acid oxidation in the liver may underlie some antiobese and lipid lowering properties of supplemental TTA

Oral supplementation of TTA in both rats[24] and humans (at 1,000mg daily for four weeks[3]) has been noted to increase the amount of oleic acid (an omega-9 fatty acid found in many foods such as Olive Oil) in the body relative to other fatty acids despite a downward trend of overall fatty acid content in the blood. This is thought to be due to the delta 9 desaturase (Δ9D) enzyme which produces oleic acid from saturated fatty acids in the body[25] being increased (independent of possible PPARα activation from TTA[26]) as TTA itself is thought to be primarily metabolized by this enzyme.

Possibly related to how TTA is metabolized by the delta-9-desaturase enzyme, this enzyme's activity may be increased which is thought to result in a higher rate of production of oleic acid from other fatty acids in the body. The end result being a higher relative content of oleic acid.

9.2. Kidneys

TTA supplementation can also alleviate damages to the kidneys and prevent subseqeunt increases in blood pressure.[24][27]

In otherwise healthy humans given TTA for a period of a week at 1,000mg no noticeable damage occurred to the kidneys as assessed by their biomarkers in the blood.[4]


Edit10. Interactions with Aesthetics

10.1. Skin

Psoriasis is an inflammatory skin disease which is characterized by an increase in the inflammatory response and its cytokines (mediators of inflammation in the body) such as TNF-α[28] resulting in an inflammatory response in the skin characterized by redness, thickness, and scaleness of the skin.[29] Due to the previously noted ability of Tetradecyl thioacetic acid (TTA) to reduce the aforemetnioned cytokine following oral administration[13] it has been investigated for the treatment of psoriasis.

A topical cream containing 0.5% tetradecyl thioacetic acid (TTA) given to psoriatic patients failed to exert any anti-psoriatic effects in a small pilot study[30] whereas elsewhere in a larger trial oral supplementation of 1,000mg TTA over the course of four weeks showed a reduction in inflammatory biomarkers in persons with psoriasis (ICAM-1, IL-8, TNF-α) but psoriasis symptoms were not directly assessed.[3]

While TTA may possess an anti-inflammatory effect, there is currently no evidence to demonstrate an ability of TTA to actively treat psoriasis. The body of evidence for this, however, is limited


Edit11. Nutrient-Nutrient Interactions

11.1. Vitamin E

28 days supplementation of TTA was able to significantly reduce circulating Vitamin E levels by about 2nM, which was said to be due to the tight relationship with serum Vitamin E and serum lipids.[1] TTA is able to slightly reduce total circulating lipids in both healthy persons[4] and moreso in those with metabolic ailment (diabetes) due to higher baseline lipids,[1] supporting this hypothesis. The reduction in serum Vitamin E, while statistically significant, does not appear to be large enough to be clinically worrisome.[1]

Due to reducing overall lipids in the blood, it is possible that TTA can reduce things that bind to lipids to transport themselves around the body. This has been demonstrated with Vitamin E and should apply to other fat soluble nutrients (CoQ10 and carotenoids) although it does not seem to be enough of a reduction to be clinically worrisome.

11.2. Fish Oil

The two main fatty acids in Fish Oil, EPA and DHA, had their circulating levels decreased by 10% and 13% respectively, after 28 days of 1g TTA supplementation in diabetic and hyperlipidemic men,[1] which the authors attributed to increase peroxisomal fatty acid oxidation favoring omega-3 polyunsaturated fatty acids, which is seen in rats.[31]


Edit12. Safety and Toxicity

12.1. General

Tetradecyl thioacetic acid (TTA) has been subject to toxicity testing based on the concern that, secondary to its fat solubility, it could be stored in the body for prolonged periods of time and have chronic effects different from acute effects. Preliminary investigation into human consumption have found no adverse effects at doses of 300-1000mg a day for 7 consecutive days in otherwise healthy adult men[4] while another study noted no side effects at 1000mg for 28 days in diabetic males.[1]

Unpublished preclinical toxicity studies in dogs and rats (unpublished, but mentioned in citation) have apparently concluded little to no risk of toxicity.[1]

No apparent toxicity at this moment in time with oral doses up to 1,000mg daily for a period of up to one month, with no data of either higher doses or longer periods of time in humans

References

  1. Løvås K, et al. Tetradecylthioacetic acid attenuates dyslipidaemia in male patients with type 2 diabetes mellitus, possibly by dual PPAR-alpha/delta activation and increased mitochondrial fatty acid oxidation. Diabetes Obes Metab. (2009)
  2. Røst TH, et al. A pan-PPAR ligand induces hepatic fatty acid oxidation in PPARalpha-/- mice possibly through PGC-1 mediated PPARdelta coactivation. Biochim Biophys Acta. (2009)
  3. Morken T, et al. Anti-inflammatory and hypolipidemic effects of the modified fatty acid tetradecylthioacetic acid in psoriasis--a pilot study. Scand J Clin Lab Invest. (2011)
  4. Pettersen RJ, et al. Pharmacology and safety of tetradecylthioacetic acid (TTA): phase-1 study. J Cardiovasc Pharmacol. (2008)
  5. Gedde-Dahl A, et al. Tetradecylthioacetic acid (a 3-thia fatty acid) decreases triacylglycerol secretion in CaCo-2 cells. J Lipid Res. (1995)
  6. Skrede S, et al. Stimulation of fatty acid oxidation by a 3-thia fatty acid reduces triacylglycerol secretion in cultured rat hepatocytes. J Lipid Res. (1994)
  7. Gedde-Dahl A, et al. Tetradecylthioacetic acid (a 3-thia fatty acid) impairs secretion of oleic acid-induced triacylglycerol-rich lipoproteins in CaCo-2 cells. Biochim Biophys Acta. (1999)
  8. Larsen LN, et al. Sulfur-substituted and alpha-methylated fatty acids as peroxisome proliferator-activated receptor activators. Lipids. (2005)
  9. Berge RK, Hvattum E. Impact of cytochrome P450 system on lipoprotein metabolism. Effect of abnormal fatty acids (3-thia fatty acids). Pharmacol Ther. (1994)
  10. Strand E, et al. Long-term treatment with the pan-PPAR agonist tetradecylthioacetic acid or fish oil is associated with increased cardiac content of n-3 fatty acids in rat. Lipids Health Dis. (2012)
  11. Whitman SC, et al. Macrophage-specific expression of class A scavenger receptors in LDL receptor(-/-) mice decreases atherosclerosis and changes spleen morphology. J Lipid Res. (2002)
  12. de Winther MP, et al. Macrophage scavenger receptor class A: A multifunctional receptor in atherosclerosis. Arterioscler Thromb Vasc Biol. (2000)
  13. Fredriksen J, et al. Lipid-lowering and anti-inflammatory effects of tetradecylthioacetic acid in HIV-infected patients on highly active antiretroviral therapy. Eur J Clin Invest. (2004)
  14. Tetradecylthioacetic acid prevents high fat diet induced adiposity and insulin resistance
  15. Wergedahl H, et al. Long term treatment with tetradecylthioacetic acid improves the antioxidant status in obese Zucker (fa/fa) rats. Drug Metab Lett. (2008)
  16. Khalid AM, et al. Cardioprotective effect of the PPAR ligand tetradecylthioacetic acid in type 2 diabetic mice. Am J Physiol Heart Circ Physiol. (2011)
  17. Hafstad AD, et al. Cardiac peroxisome proliferator-activated receptor-alpha activation causes increased fatty acid oxidation, reducing efficiency and post-ischaemic functional loss. Cardiovasc Res. (2009)
  18. Madsen L, et al. Tetradecylthioacetic acid prevents high fat diet induced adiposity and insulin resistance. J Lipid Res. (2002)
  19. Rao MS, Reddy JK. Peroxisomal beta-oxidation and steatohepatitis. Semin Liver Dis. (2001)
  20. Wensaas AJ, et al. Dietary supplementation of tetradecylthioacetic acid increases feed intake but reduces body weight gain and adipose depot sizes in rats fed on high-fat diets. Diabetes Obes Metab. (2009)
  21. Burri L, et al. Tetradecylthioacetic acid increases hepatic mitochondrial β-oxidation and alters fatty acid composition in a mouse model of chronic inflammation. Lipids. (2011)
  22. Berge RK, et al. Metabolic effects of thia fatty acids. Curr Opin Lipidol. (2002)
  23. Hagland HR, et al. Induction of mitochondrial biogenesis and respiration is associated with mTOR regulation in hepatocytes of rats treated with the pan-PPAR activator tetradecylthioacetic acid (TTA). Biochem Biophys Res Commun. (2013)
  24. Gudbrandsen OA, et al. Prevention of hypertension and organ damage in 2-kidney, 1-clip rats by tetradecylthioacetic acid. Hypertension. (2006)
  25. Up-regulated delta 9-desaturase gene expression by hypolipidemic peroxisome-proliferating fatty acids results in increased oleic acid content in liver and VLDL: accumulation of a delta 9-desaturated metabolite of tetradecylthioacetic acid
  26. Up-regulated delta 9-desaturase gene expression by hypolipidemic peroxisome-proliferating fatty acids results in increased oleic acid content in liver and VLDL: accumulation of a delta 9-desaturated metabolite of tetradecylthioacetic acid
  27. Tetradecylthioacetic acid prevents the inflammatory response in two-kidney, one-clip hypertension
  28. Ogilvie AL, et al. Leukocyte infiltration and mRNA expression of IL-20, IL-8 and TNF-R P60 in psoriatic skin is driven by TNF-alpha. Int J Immunopathol Pharmacol. (2006)
  29. Mason A, et al. Topical treatments for chronic plaque psoriasis: an abridged Cochrane systematic review. J Am Acad Dermatol. (2013)
  30. Kuenzli S, Saurat JH. Effect of topical PPARbeta/delta and PPARgamma agonists on plaque psoriasis. A pilot study. Dermatology. (2003)
  31. Madsen L, et al. Docosahexaenoic and eicosapentaenoic acids are differently metabolized in rat liver during mitochondria and peroxisome proliferation. J Lipid Res. (1998)

(Common misspellings for Tetradecyl Thioacetic Acid include tetradecal, tetradecil, tetradekil, tetradekyl, theoacetic, thioacitic, theoacidic)

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